CB1-cannabinoid receptors are involved in the modulation of non-synaptic [3H]serotonin release from the rat hippocampus

Tamás Balázsa, Judit Bíró, Nóra Gullai, Catherine Ledent, B. Sperlágh

Research output: Contribution to journalArticle

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Abstract

In the present study we investigated whether serotonin release in the hippocampus is subject to regulation via cannabinoid receptors. Both rat and mouse hippocampal slices were preincubated with [3H]serotonin ([3H]5-HT) and superfused with medium containing serotonin reuptake inhibitor citalopram hydrobromide (300 nM). The cannabinoid receptor agonist R(+)-[2,3-dihydro-5-methyl-3-[(morpholinyl)methyl]pyrrolo[1,2,3-de]-1,4-benzoxazinyl]-(1-naphthalenyl) methanone mesylate (WIN55,212-2, 1 μM) did not affect either the resting or the electrically evoked [3H]5-HT release. In the presence of the ionotropic glutamate receptor antagonists d(-)-2-amino-5-phosphonopentanoic acid (AP-5, 50 μM) and 6-cyano-7-nitroquinoxaline-2,3-dione-disodium (CNQX, 10 μM) the evoked [3H]5-HT release was decreased significantly. Similar findings were obtained when CNQX (10 μM) was applied alone with WIN55,212-2. This effect was abolished by the selective cannabinoid receptor subtype 1 (CB1) antagonists N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (SR141716, 1 μM) and 1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-1-piperidinyl-1H-pyrazole-3-carboxamide trifluoroacetate salt (AM251, 1 μM). Similarly to that observed in rats, WIN55,212-2 (1 μM) decreased the evoked [3H]5-HT efflux in wild-type mice (C B1+ / +). The inhibitory effect of WIN55,212-2 (1 μM) was completely absent in hippocampal slices derived from mice genetically deficient in CB1 cannabinoid receptors (C B1- / -). Relatively selective degeneration of fine serotonergic axons by the neurotoxin parachloramphetamine (PCA) reduced significantly the tritium uptake and the evoked [3H]5-HT release. In addition, PCA, eliminated the effect of WIN55,212-2 (1 μM) on the stimulation-evoked [3H]5-HT efflux. In contrast to the PCA-treated animals, WIN55,212-2 (1 μM) reduced the [3H]5-HT efflux in the saline-treated group. Our data suggest that a subpopulation of non-synaptic serotonergic afferents express CB1 receptors and activation of these CB1 receptors leads to a decrease in 5-HT release.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalNeurochemistry International
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Cannabinoid Receptor CB1
Hippocampus
Serotonin
6-Cyano-7-nitroquinoxaline-2,3-dione
Cannabinoid Receptors
rimonabant
Cannabinoid Receptor Agonists
2-Amino-5-phosphonovalerate
Ionotropic Glutamate Receptors
Trifluoroacetic Acid
Excitatory Amino Acid Antagonists
Citalopram
Mesylates
Tritium
Neurotoxins
Serotonin Uptake Inhibitors
Axons
Salts

Keywords

  • CB receptor
  • Hippocampus
  • Inhibition
  • Parachloroamphetamine
  • Release
  • Serotonin

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

CB1-cannabinoid receptors are involved in the modulation of non-synaptic [3H]serotonin release from the rat hippocampus. / Balázsa, Tamás; Bíró, Judit; Gullai, Nóra; Ledent, Catherine; Sperlágh, B.

In: Neurochemistry International, Vol. 52, No. 1, 01.2008, p. 95-102.

Research output: Contribution to journalArticle

Balázsa, Tamás ; Bíró, Judit ; Gullai, Nóra ; Ledent, Catherine ; Sperlágh, B. / CB1-cannabinoid receptors are involved in the modulation of non-synaptic [3H]serotonin release from the rat hippocampus. In: Neurochemistry International. 2008 ; Vol. 52, No. 1. pp. 95-102.
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N2 - In the present study we investigated whether serotonin release in the hippocampus is subject to regulation via cannabinoid receptors. Both rat and mouse hippocampal slices were preincubated with [3H]serotonin ([3H]5-HT) and superfused with medium containing serotonin reuptake inhibitor citalopram hydrobromide (300 nM). The cannabinoid receptor agonist R(+)-[2,3-dihydro-5-methyl-3-[(morpholinyl)methyl]pyrrolo[1,2,3-de]-1,4-benzoxazinyl]-(1-naphthalenyl) methanone mesylate (WIN55,212-2, 1 μM) did not affect either the resting or the electrically evoked [3H]5-HT release. In the presence of the ionotropic glutamate receptor antagonists d(-)-2-amino-5-phosphonopentanoic acid (AP-5, 50 μM) and 6-cyano-7-nitroquinoxaline-2,3-dione-disodium (CNQX, 10 μM) the evoked [3H]5-HT release was decreased significantly. Similar findings were obtained when CNQX (10 μM) was applied alone with WIN55,212-2. This effect was abolished by the selective cannabinoid receptor subtype 1 (CB1) antagonists N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (SR141716, 1 μM) and 1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-1-piperidinyl-1H-pyrazole-3-carboxamide trifluoroacetate salt (AM251, 1 μM). Similarly to that observed in rats, WIN55,212-2 (1 μM) decreased the evoked [3H]5-HT efflux in wild-type mice (C B1+ / +). The inhibitory effect of WIN55,212-2 (1 μM) was completely absent in hippocampal slices derived from mice genetically deficient in CB1 cannabinoid receptors (C B1- / -). Relatively selective degeneration of fine serotonergic axons by the neurotoxin parachloramphetamine (PCA) reduced significantly the tritium uptake and the evoked [3H]5-HT release. In addition, PCA, eliminated the effect of WIN55,212-2 (1 μM) on the stimulation-evoked [3H]5-HT efflux. In contrast to the PCA-treated animals, WIN55,212-2 (1 μM) reduced the [3H]5-HT efflux in the saline-treated group. Our data suggest that a subpopulation of non-synaptic serotonergic afferents express CB1 receptors and activation of these CB1 receptors leads to a decrease in 5-HT release.

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