Caveolin isoforms in resident and elicited rat peritoneal macrophages

A. Kiss, Agnes Túri, Nándor Müllner, J. Tímár

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Caveolin - an integral membrane protein - is the principal component of caveolae membranes in vivo. Multiple forms of caveolin have been identified: caveolin-1α, caveolin-1β, caveolin-2 and caveolin-3. They differ in their specific properties and tissue distribution. When we studied the lysate of resident and elicited macrophages isolated from rat peritoneal cavity by Western blot analysis, we identified two different proteins (~ 29 kDa and ~ 20 kDa) which were labelled with anti-caveolin antibodies. The ~ 20-kDa protein was labelled specifically only by anti-VIP21/caveolin-1, while the ~ 29-kDa protein was labelled by anti-VIP21/caveolin-1 and anti-caveolin-2. The presence of the ~ 29-kDa protein was characteristic of resident macrophages, and only a small amount of the ~ 20-kDa protein was detected in these cells. Elicitation resulted in a significant increase in the amount of the ~ 20-kDa protein labelled by anti-VIP21/caveolin-1 only. According to its molecular mass and antibody-specificity, this protein might be identical with the caveolin-1β isoform. Our morphological (confocal and electron microscopical) studies have shown that in resident cells caveolin was present in the cytoplasm, in smaller vesicles and multivesicular bodies around the Golgi area. Only a very small amount of caveolae was found on the surface of these cells. In elicited macrophages, caveolae (labelled with the anti-VIP21/caveolin-1 antibody) appeared in large numbers on the cell surface, but caveolin detected by anti-caveolin-2 was also found in small vesicles and multivesicular bodies in the cytoplasm. According to these results, the absence of caveolae in resident cells can be explained by the absence of caveolin-1. The expression of the ~ 29-kDa (caveolin-related) protein in resident macrophages seems to be insufficient for caveolae formation. Elicitation significantly increased the expression of caveolin-1, and the increased amount of caveolin-1 resulted in caveolae formation on the cell surface.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalEuropean Journal of Cell Biology
Volume79
Issue number5
Publication statusPublished - 2000

Fingerprint

Caveolins
Caveolin 1
Peritoneal Macrophages
Protein Isoforms
Caveolae
Caveolin 2
Macrophages
Proteins
Multivesicular Bodies
Cytoplasm
Caveolin 3
Antibody Specificity
Peritoneal Cavity
Tissue Distribution
Anti-Idiotypic Antibodies
Membrane Proteins
Cell Count
Western Blotting
Electrons

Keywords

  • Caveolae
  • Caveolin isoforms
  • Elicited macrophages
  • Resident macrophages

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Caveolin isoforms in resident and elicited rat peritoneal macrophages. / Kiss, A.; Túri, Agnes; Müllner, Nándor; Tímár, J.

In: European Journal of Cell Biology, Vol. 79, No. 5, 2000, p. 343-349.

Research output: Contribution to journalArticle

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