Caveolin-1 is transported to multi-vesicular bodies after albumin-induced endocytosis of caveolae in HepG2 cells

E. Botos, J. Klumperman, V. Oorschot, B. Ígyártó, A. Magyar, M. Oláh, A. Kiss

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Caveolae-mediated endocytosis is a highly regulated endocytic pathway that exists in parallel to other forms of clathrin-dependent and -independent endocytosis. Internalized caveolae accumulate in intermediate organelles called caveosomes. Here we addressed the further fate of internalized caveolae by inducing caveolae-mediated uptake of albumin by HepG2 cells. We followed the route of internalized caveolin-1 by immunogold labelling of ultrathin frozen sections and by Western blot analyses of purified membrane fractions. Long-term (1 and 3 hrs) albumin treatment resulted in the appearance of albumin-containing caveolae in special multi-caveolar complexes (consisting of multiple caveolae clustered together) connected to the plasma membrane and caveosome-like structures in the cytoplasm. In addition, numerous CD63 (LIMP-1) positive late endosomes/multi-vesicular bodies were found positive for caveolin-1, suggesting that upon albumin incubation, caveolin-1 is endocytosed and enters the degradative pathway. Surprisingly, the number of caveolae at the plasma membrane increased after addition of albumin. This increase was blocked by cycloheximide treatment, indicating that albumin internalization also stimulates de novo protein synthesis, which is necessary for new caveolae formation. Together, our results show that during long-term albumin uptake, caveolin-1 travels to late endosomes and is replaced by newly synthesized caveolin-1 at the plasma membrane.

Original languageEnglish
Pages (from-to)1632-1639
Number of pages8
JournalJournal of Cellular and Molecular Medicine
Volume12
Issue number5A
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Multivesicular Bodies
Caveolin 1
Caveolae
Hep G2 Cells
Endocytosis
Albumins
Endosomes
Cell Membrane
CD63 Antigen
Clathrin
Frozen Sections
Cycloheximide
Organelles
Cytoplasm
Western Blotting
Membranes

Keywords

  • Albumin
  • Caveolin-1
  • Multi-vesicular bodies

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Caveolin-1 is transported to multi-vesicular bodies after albumin-induced endocytosis of caveolae in HepG2 cells. / Botos, E.; Klumperman, J.; Oorschot, V.; Ígyártó, B.; Magyar, A.; Oláh, M.; Kiss, A.

In: Journal of Cellular and Molecular Medicine, Vol. 12, No. 5A, 09.2008, p. 1632-1639.

Research output: Contribution to journalArticle

Botos, E. ; Klumperman, J. ; Oorschot, V. ; Ígyártó, B. ; Magyar, A. ; Oláh, M. ; Kiss, A. / Caveolin-1 is transported to multi-vesicular bodies after albumin-induced endocytosis of caveolae in HepG2 cells. In: Journal of Cellular and Molecular Medicine. 2008 ; Vol. 12, No. 5A. pp. 1632-1639.
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