Cationic permethylated 6-monoamino-6-monodeoxy-β-cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis

Krisztina Németh, Celesztina Domonkos, Virág Sarnyai, Julianna Szemán, László Jicsinszky, Lajos Szente, Júlia Visy

Research output: Contribution to journalArticle

7 Citations (Scopus)


The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) - a single isomer, cationic CD derivative - developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - Oct 2014



  • Cationic CD derivative
  • Dansylated amino acid
  • Enantioseparation
  • Isoelectric point
  • Lipophilicity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this