Cationic permethylated 6-monoamino-6-monodeoxy-β-cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis

Krisztina Németh, Celesztina Domonkos, Virág Sarnyai, Julianna Szemán, László Jicsinszky, Lajos Szente, J. Visy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) - a single isomer, cationic CD derivative - developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume99
DOIs
Publication statusPublished - 2014

Fingerprint

Capillary electrophoresis
Cyclodextrins
Capillary Electrophoresis
Amino Acids
Isoelectric Point
Isomers
Chemical properties
Derivatives

Keywords

  • Cationic CD derivative
  • Dansylated amino acid
  • Enantioseparation
  • Isoelectric point
  • Lipophilicity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry
  • Medicine(all)

Cite this

Cationic permethylated 6-monoamino-6-monodeoxy-β-cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis. / Németh, Krisztina; Domonkos, Celesztina; Sarnyai, Virág; Szemán, Julianna; Jicsinszky, László; Szente, Lajos; Visy, J.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 99, 2014, p. 16-21.

Research output: Contribution to journalArticle

Németh, Krisztina ; Domonkos, Celesztina ; Sarnyai, Virág ; Szemán, Julianna ; Jicsinszky, László ; Szente, Lajos ; Visy, J. / Cationic permethylated 6-monoamino-6-monodeoxy-β-cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis. In: Journal of Pharmaceutical and Biomedical Analysis. 2014 ; Vol. 99. pp. 16-21.
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AU - Sarnyai, Virág

AU - Szemán, Julianna

AU - Jicsinszky, László

AU - Szente, Lajos

AU - Visy, J.

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