Cation and anion transport pathways in volume regulatory response of human lymphocytes to hyposmotic media

B. Sarkadi, R. Cheung, E. Mack

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The regulatory volume decrease of osmotically swollen human peripheral blood lymphocytes can be inhibited by agents acting on volume-activated K+- or Cl--transport pathways. Quinine, cetiedil, and 3,3'-dipropylthiadicarboxyanine were found to block the volume-induced K+ transport by interaction with sites on the outside face of the membrane, perhaps by competition with external K+. Drugs known to influence calmodulin action inhibit both volume-induced K+ and Cl- transport to varying degrees. Those inhibitors, particularly of K+ transport, are correlated with their calmodulin-antagonist activity. Penetrating sulfhydryl (SH) reagents (in contrast to nonpenetrating ones) are potent inhibitors of both volume-induced K+ and Cl- movements, indicating the presence of functionally important SH groups located within the membrane or at the cytoplasmic face. A number of agents, such as dipyridamole and oligomycin C, are specific inhibitors of the volume-activated anion pathway. In all respects studied, the inhibition characteristics of the volume-activated K+ pathway of lymphocytes resemble those of the Ca2+-activated K+ channel of red cells. In contrast, the volume-induced anion permeability differs from the primary anion-transport pathway of red cells.

Original languageEnglish
Pages (from-to)C480-C487
JournalAmerican Journal of Physiology - Cell Physiology
Volume17
Issue number3
DOIs
Publication statusPublished - Jan 1 1985

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Fingerprint Dive into the research topics of 'Cation and anion transport pathways in volume regulatory response of human lymphocytes to hyposmotic media'. Together they form a unique fingerprint.

  • Cite this