Catecholamines released from local adrenergic axon terminals are possibly involved in fine tuning of steroid secretion from zona glomerulosa cells: Functional and morphological evidence

E. Vízi, I. Tóth, K. S. Szalay, K. Windisch, E. Orso, D. Szabo, G. P. Vinson

Research output: Contribution to journalArticle

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Abstract

The effect of supramaximal electric field stimulation on [3H]noradrenaline (NA) release and hormone production by rat adrenal capsule-glomerulosa preparations was studied using a microvolume perfusion system. A substantial proportion (about 20%) of nerve endings (varicosities) were observed close to zona glomerulosa cells, and about half of them appeared to be catecholaminergic, as judged by the chromaffin reaction of the synaptic vesicles studied at electron microscopic level. In tissue, preloaded with [3H]NA, the release of NA in response to electrical stimulation was frequency-dependent. Reserpinization, calcium removal or inhibition of Na+influx by tetrodotoxin completely blocked NA release by field stimulation, indicating that the release resulted from axonal activity and is of vesicular origin. Neither the α2-adrenoceptor agonist xylazine nor the muscarine-receptor agonist oxotremorine affected the stimulation-evoked release of [3H]NA, suggesting that, in contrast with other neurones present in the central nervous system or in the peripheral autonomic nervous system but like those in the median eminence, these axon terminals contained few presynaptic modulatory receptors. The NA (10.20 ± 1.79 (S.E.M.) μg/g, n = 9), adrenaline (24.38 ± 5.50 μg/g, n = 9) and dopamine (0.35 ± 0.09 μg/g, n = 6) contents of the preparations were high, as determined by high-performance liquid chromatography. Our observations that the release and content of NA is high, and that a substantial proportion of catecholaminergic axon terminals lie in close proximity to zona glomerulosa cells (median value of the distance 300 nm) or to smooth muscle cells of the vessels, suggest that NA released from local adrenergic neurones without being presynaptically modulated may play an important role in fine tuning both steroid production and/or blood flow through the gland, itself a powerful modulator of the adrenocortical response. This local modulating effect of NA may be especially significant when sympathetic activity is enhanced.

Original languageEnglish
Pages (from-to)551-561
Number of pages11
JournalJournal of Endocrinology
Volume135
Issue number3
Publication statusPublished - 1992

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Zona Glomerulosa
Presynaptic Terminals
Adrenergic Agents
Catecholamines
Norepinephrine
Steroids
Electric Stimulation
Muscarine
Oxotremorine
Presynaptic Receptors
Xylazine
Adrenergic Neurons
Median Eminence
Nerve Endings
Synaptic Vesicles
Autonomic Nervous System
Tetrodotoxin
Peripheral Nervous System
Adrenergic Receptors
Epinephrine

ASJC Scopus subject areas

  • Endocrinology

Cite this

Catecholamines released from local adrenergic axon terminals are possibly involved in fine tuning of steroid secretion from zona glomerulosa cells : Functional and morphological evidence. / Vízi, E.; Tóth, I.; Szalay, K. S.; Windisch, K.; Orso, E.; Szabo, D.; Vinson, G. P.

In: Journal of Endocrinology, Vol. 135, No. 3, 1992, p. 551-561.

Research output: Contribution to journalArticle

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AU - Vízi, E.

AU - Tóth, I.

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AU - Vinson, G. P.

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