Catalytic hydrogenation of polyunsaturated biological membranes: Effects on membrane fatty acid composition and physical properties

James A. Logue, L. Vígh, Ferenc Joó, Andrew R. Cossins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The relationship between phospholipid saturation and membrane physical structure in a complex, highly polyunsaturated biological membrane (trout liver microsomes) has been studied by the graded and specific hydrogenation of polyunsaturated fatty acids. The homogeneous catalyst Pd(QS)2 caused rapid and effective hydrogenation, increasing the proportion of saturated fatty acids from 20-30% up to 60%, without loss or fragmentation. Long chain, polyunsaturated fatty acids (20:5ω3, 22:6ω3) were rapidly converted to a large number of partially hydrogenated isomers, and ultimately to the fully saturated C20 or C22 fatty acids. C18 mono- and di-unsaturates showed slower rates of hydrogenation. Increased saturation was closely associated with an increased membrane physical order as determined by the fluorescence anisotropy probe, 1,6-diphenyl-1,3,5-hexatriene. However, extensive hydrogenation led to highly ordered membranes exhibiting a gel-liquid crystalline phase transition between 30 and 60°C. Polyunsaturated membranes can thus be converted into partially or substantially saturated membranes with measurable phase structure without direct alteration of other membrane components. This offers a less equivocal means of assessing the influence of polyunsaturation upon membrane structure and function.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalBBA - Biomembranes
Volume1368
Issue number1
DOIs
Publication statusPublished - Jan 5 1998

Fingerprint

Biological membranes
Hydrogenation
Fatty Acids
Physical properties
Membranes
Chemical analysis
Unsaturated Fatty Acids
Diphenylhexatriene
Membrane structures
Phase structure
Isomers
Liver
Fluorescence Polarization
Trout
Phase Transition
Phospholipids
Liver Microsomes
Anisotropy
Phase transitions
Gels

Keywords

  • Catalytic hydrogenation
  • Fatty acid saturation
  • Fish membrane
  • Lipid hydrogenation
  • Membrane fluidity
  • Polyunsaturated fatty acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Catalytic hydrogenation of polyunsaturated biological membranes : Effects on membrane fatty acid composition and physical properties. / Logue, James A.; Vígh, L.; Joó, Ferenc; Cossins, Andrew R.

In: BBA - Biomembranes, Vol. 1368, No. 1, 05.01.1998, p. 41-51.

Research output: Contribution to journalArticle

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