Abstract
The relationship between phospholipid saturation and membrane physical structure in a complex, highly polyunsaturated biological membrane (trout liver microsomes) has been studied by the graded and specific hydrogenation of polyunsaturated fatty acids. The homogeneous catalyst Pd(QS)2 caused rapid and effective hydrogenation, increasing the proportion of saturated fatty acids from 20-30% up to 60%, without loss or fragmentation. Long chain, polyunsaturated fatty acids (20:5ω3, 22:6ω3) were rapidly converted to a large number of partially hydrogenated isomers, and ultimately to the fully saturated C20 or C22 fatty acids. C18 mono- and di-unsaturates showed slower rates of hydrogenation. Increased saturation was closely associated with an increased membrane physical order as determined by the fluorescence anisotropy probe, 1,6-diphenyl-1,3,5-hexatriene. However, extensive hydrogenation led to highly ordered membranes exhibiting a gel-liquid crystalline phase transition between 30 and 60°C. Polyunsaturated membranes can thus be converted into partially or substantially saturated membranes with measurable phase structure without direct alteration of other membrane components. This offers a less equivocal means of assessing the influence of polyunsaturation upon membrane structure and function.
| Original language | English |
|---|---|
| Pages (from-to) | 41-51 |
| Number of pages | 11 |
| Journal | BBA - Biomembranes |
| Volume | 1368 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 5 1998 |
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Keywords
- Catalytic hydrogenation
- Fatty acid saturation
- Fish membrane
- Lipid hydrogenation
- Membrane fluidity
- Polyunsaturated fatty acid
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biophysics
Cite this
Catalytic hydrogenation of polyunsaturated biological membranes : Effects on membrane fatty acid composition and physical properties. / Logue, James A.; Vígh, L.; Joó, Ferenc; Cossins, Andrew R.
In: BBA - Biomembranes, Vol. 1368, No. 1, 05.01.1998, p. 41-51.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Catalytic hydrogenation of polyunsaturated biological membranes
T2 - Effects on membrane fatty acid composition and physical properties
AU - Logue, James A.
AU - Vígh, L.
AU - Joó, Ferenc
AU - Cossins, Andrew R.
PY - 1998/1/5
Y1 - 1998/1/5
N2 - The relationship between phospholipid saturation and membrane physical structure in a complex, highly polyunsaturated biological membrane (trout liver microsomes) has been studied by the graded and specific hydrogenation of polyunsaturated fatty acids. The homogeneous catalyst Pd(QS)2 caused rapid and effective hydrogenation, increasing the proportion of saturated fatty acids from 20-30% up to 60%, without loss or fragmentation. Long chain, polyunsaturated fatty acids (20:5ω3, 22:6ω3) were rapidly converted to a large number of partially hydrogenated isomers, and ultimately to the fully saturated C20 or C22 fatty acids. C18 mono- and di-unsaturates showed slower rates of hydrogenation. Increased saturation was closely associated with an increased membrane physical order as determined by the fluorescence anisotropy probe, 1,6-diphenyl-1,3,5-hexatriene. However, extensive hydrogenation led to highly ordered membranes exhibiting a gel-liquid crystalline phase transition between 30 and 60°C. Polyunsaturated membranes can thus be converted into partially or substantially saturated membranes with measurable phase structure without direct alteration of other membrane components. This offers a less equivocal means of assessing the influence of polyunsaturation upon membrane structure and function.
AB - The relationship between phospholipid saturation and membrane physical structure in a complex, highly polyunsaturated biological membrane (trout liver microsomes) has been studied by the graded and specific hydrogenation of polyunsaturated fatty acids. The homogeneous catalyst Pd(QS)2 caused rapid and effective hydrogenation, increasing the proportion of saturated fatty acids from 20-30% up to 60%, without loss or fragmentation. Long chain, polyunsaturated fatty acids (20:5ω3, 22:6ω3) were rapidly converted to a large number of partially hydrogenated isomers, and ultimately to the fully saturated C20 or C22 fatty acids. C18 mono- and di-unsaturates showed slower rates of hydrogenation. Increased saturation was closely associated with an increased membrane physical order as determined by the fluorescence anisotropy probe, 1,6-diphenyl-1,3,5-hexatriene. However, extensive hydrogenation led to highly ordered membranes exhibiting a gel-liquid crystalline phase transition between 30 and 60°C. Polyunsaturated membranes can thus be converted into partially or substantially saturated membranes with measurable phase structure without direct alteration of other membrane components. This offers a less equivocal means of assessing the influence of polyunsaturation upon membrane structure and function.
KW - Catalytic hydrogenation
KW - Fatty acid saturation
KW - Fish membrane
KW - Lipid hydrogenation
KW - Membrane fluidity
KW - Polyunsaturated fatty acid
UR - http://www.scopus.com/inward/record.url?scp=0032484657&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032484657&partnerID=8YFLogxK
U2 - 10.1016/S0005-2736(97)00203-4
DO - 10.1016/S0005-2736(97)00203-4
M3 - Article
C2 - 9459583
AN - SCOPUS:0032484657
VL - 1368
SP - 41
EP - 51
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -