Ca2+ imaging of mouse neocortical interneurone dendrites

Ia-type K+ channels control action potential backpropagation

Jesse H. Goldberg, G. Tamás, Rafael Yuste

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

GABAergic interneurones are essential in cortical processing, yet the functional properties of their dendrites are still poorly understood. In this first study, we combined two-photon calcium imaging with whole-cell recording and anatomical reconstructions to examine the calcium dynamics during action potential (AP) backpropagation in three types of V1 supragranular interneurones: parvalbumin-positive fast spikers (FS), calretinin-positive irregular spikers (IS), and adapting cells (AD). Somatically generated APs actively backpropagated into the dendritic tree and evoked instantaneous calcium accumulations. Although voltage-gated calcium channels were expressed throughout the dendritic arbor, calcium signals during backpropagation of both single APs and AP trains were restricted to proximal dendrites. This spatial control of AP backpropagation was mediated by Ia-type potassium currents and could be mitigated by by previous synaptic activity. Further, we observed supralinear summation of calcium signals in synaptically activated dendritic compartments. Together, these findings indicate that in interneurons, dendritic AP propagation is synaptically regulated. We propose that interneurones have a perisomatic and a distal dendritic functional compartment, with different integrative functions.

Original languageEnglish
Pages (from-to)49-65
Number of pages17
JournalJournal of Physiology
Volume551
Issue number1
DOIs
Publication statusPublished - Aug 15 2003

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Interneurons
Dendrites
Action Potentials
Calcium
Calbindin 2
Parvalbumins
Patch-Clamp Techniques
Calcium Channels
Photons
Potassium

ASJC Scopus subject areas

  • Physiology

Cite this

Ca2+ imaging of mouse neocortical interneurone dendrites : Ia-type K+ channels control action potential backpropagation. / Goldberg, Jesse H.; Tamás, G.; Yuste, Rafael.

In: Journal of Physiology, Vol. 551, No. 1, 15.08.2003, p. 49-65.

Research output: Contribution to journalArticle

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