Ca2+-dependent control of the permeability properties of the mitochondrial outer membrane and voltage-dependent anion-selective channel (VDAC)

G. Báthori, György Csordás, Cecilia Garcia-Perez, Erika Davies, György Hajnóczky

Research output: Contribution to journalArticle

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Abstract

Cell function depends on the distribution of cytosolic and mitochondrial factors across the outer mitochondrial membrane (OMM). Passage of metabolites through the OMM has been attributed to the voltage-dependent anion-selective channel (VDAC), which can form a large conductance and permanently open a channel in lipid bilayers. However, recent data indicate that the transport of metabolites through the OMM is controlled in the cells. Recognizing that the bilayer studies had been commonly conducted at supraphysiological [Ca 2+] and [K+], we determined the effect of Ca2+ on VDAC activity. In liposomes, the purified VDAC displays Ca 2+-dependent control of the molecular cut-off size and shows Ca 2+-regulated Ca2+ permeability in the physiological [Ca2+] range. In bilayer experiments, at submicromolar [Ca 2+], the purified VDAC or isolated OMM does not show sustained large conductance but rather exhibits gating between a nonconducting state and various subconductance states. Ca2+ addition causes a reversible increase in the conductance and may evoke channel opening to full conductance. Furthermore, single cell imaging data indicate that Ca2+ may facilitate the cation and ATP transport across the OMM. Thus, the VDAC gating is dependent on the physiological concentrations of cations, allowing the OMM to control the passage of ions and some small molecules. The OMM barrier is likely to decrease during the calcium signal.

Original languageEnglish
Pages (from-to)17347-17358
Number of pages12
JournalJournal of Biological Chemistry
Volume281
Issue number25
DOIs
Publication statusPublished - Jun 23 2006

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Voltage-Dependent Anion Channels
Mitochondrial Membranes
Permeability
Membranes
Metabolites
Cations
Lipid bilayers
Lipid Bilayers
Liposomes
Adenosine Triphosphate
Display devices
Ions
Calcium
Imaging techniques
Molecules

ASJC Scopus subject areas

  • Biochemistry

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Ca2+-dependent control of the permeability properties of the mitochondrial outer membrane and voltage-dependent anion-selective channel (VDAC). / Báthori, G.; Csordás, György; Garcia-Perez, Cecilia; Davies, Erika; Hajnóczky, György.

In: Journal of Biological Chemistry, Vol. 281, No. 25, 23.06.2006, p. 17347-17358.

Research output: Contribution to journalArticle

Báthori, G. ; Csordás, György ; Garcia-Perez, Cecilia ; Davies, Erika ; Hajnóczky, György. / Ca2+-dependent control of the permeability properties of the mitochondrial outer membrane and voltage-dependent anion-selective channel (VDAC). In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 25. pp. 17347-17358.
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