Casein kinases I and II bound to pig brain microtubules

Vladimir V. Risnik, Géza Ádám, Nikolay B. Gusev, P. Friedrich

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

1. Microtubules prepared from pig brain by two cycles of assembly-disassembly comprise cyclic nucleotide-independent protein kinase activity with phosvitin and troponin T as substrates. 2. Phosphocellulose chromatography resolved two phosvitin kinase activity peaks, one of which coincided with the troponin T kinase peak. 3. The activity peak corresponding to troponin T kinase was inhibited by heparin (I50 = 0.06 μg/ml), whereas the other phosvitin kinase peak was unaffected. 4. Both kinase fractions phosphorylated tubulin and microtubule-associated protein (MAP-2). 5. It is concluded that pig brain microtubules contain bound casein kinases I and II. The association may target the action of these kinases toward microtubular proteins in vivo.

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalCellular and Molecular Neurobiology
Volume8
Issue number3
DOIs
Publication statusPublished - Sep 1988

Fingerprint

Casein Kinase I
Casein Kinase II
Microtubules
Brain
Phosphotransferases
Swine
Phosvitin
Troponin T
Microtubule-Associated Proteins
Cyclic Nucleotides
Tubulin
Chromatography
Protein Kinases
Heparin
Association reactions
Substrates

Keywords

  • casein kinases
  • microtuble-associated protein (MAP-2)
  • microtubules
  • protein phosphorylation
  • tubulin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Casein kinases I and II bound to pig brain microtubules. / Risnik, Vladimir V.; Ádám, Géza; Gusev, Nikolay B.; Friedrich, P.

In: Cellular and Molecular Neurobiology, Vol. 8, No. 3, 09.1988, p. 315-324.

Research output: Contribution to journalArticle

Risnik, Vladimir V. ; Ádám, Géza ; Gusev, Nikolay B. ; Friedrich, P. / Casein kinases I and II bound to pig brain microtubules. In: Cellular and Molecular Neurobiology. 1988 ; Vol. 8, No. 3. pp. 315-324.
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