Carotenoid composition of three bloom-forming algae species

J. Deli, S. Gonda, L. Z S Nagy, I. Szabó, G. Gulyás-Fekete, A. Agócs, K. Marton, G. Vasas

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Algal mass productions are widespread events throughout the world. Most research deals with the environmental impact, ecology and toxicity of these phenomena, but the algae are also promising sources of bioactive natural products, and also are potential food additives. In the current study, we aimed to characterize the carotenoid composition of three non-toxic algal isolates with distinct taxonomical position, namely Dunaliella salina, Euglena sanguinea and a Nostoc strain.The strains were screened for carotenoid composition with HPLC-UV-APCI-MS with comparison with authentic standards. Carotenoids were purified with open column chromatography, and characterized by LC-UV-MS and NMR.All three species contained a high amount of carotenoids. The composition of the carotenoid pattern was somewhat different compared to literature data. The Dunaliella carotenoid fraction contained lutein (52.1% of total carotenoid), β-carotene (13.1%), violaxanthin (11.5%) and neoxanthin (6.2%) as chief compounds. The chief carotenoids in the Nostoc strain was echinenone (34.9%), while the main components of the Euglena isolate were diatoxanthin (39.0%), lutein (23.7%), an unidentified carotenoid (9.6%) and β-carotene (5.4%). Diatoxanthin is identified by NMR spectroscopy.The carotenoid patterns of the examined strains are somewhat different from the patterns described in the literature. This can be the results of genetic or environmental differences, or combinations thereof. Despite these differences, our study shows the potency of these algae in production of carotenoids, and possibly, usage as food additives.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalFood Research International
Volume65
Issue numberPB
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

algae
Carotenoids
carotenoids
Euglena
Nostoc
Lutein
Food Additives
food additives
carotenes
lutein
Dunaliella salina
neoxanthin
Dunaliella
violaxanthin

Keywords

  • Carotenoids
  • HPLC
  • HPLC-MS
  • Microalgae

ASJC Scopus subject areas

  • Food Science

Cite this

Carotenoid composition of three bloom-forming algae species. / Deli, J.; Gonda, S.; Nagy, L. Z S; Szabó, I.; Gulyás-Fekete, G.; Agócs, A.; Marton, K.; Vasas, G.

In: Food Research International, Vol. 65, No. PB, 01.11.2014, p. 215-223.

Research output: Contribution to journalArticle

Deli, J, Gonda, S, Nagy, LZS, Szabó, I, Gulyás-Fekete, G, Agócs, A, Marton, K & Vasas, G 2014, 'Carotenoid composition of three bloom-forming algae species', Food Research International, vol. 65, no. PB, pp. 215-223. https://doi.org/10.1016/j.foodres.2014.05.020
Deli J, Gonda S, Nagy LZS, Szabó I, Gulyás-Fekete G, Agócs A et al. Carotenoid composition of three bloom-forming algae species. Food Research International. 2014 Nov 1;65(PB):215-223. https://doi.org/10.1016/j.foodres.2014.05.020
Deli, J. ; Gonda, S. ; Nagy, L. Z S ; Szabó, I. ; Gulyás-Fekete, G. ; Agócs, A. ; Marton, K. ; Vasas, G. / Carotenoid composition of three bloom-forming algae species. In: Food Research International. 2014 ; Vol. 65, No. PB. pp. 215-223.
@article{1c2435a71689421f924ea6affdea4ca0,
title = "Carotenoid composition of three bloom-forming algae species",
abstract = "Algal mass productions are widespread events throughout the world. Most research deals with the environmental impact, ecology and toxicity of these phenomena, but the algae are also promising sources of bioactive natural products, and also are potential food additives. In the current study, we aimed to characterize the carotenoid composition of three non-toxic algal isolates with distinct taxonomical position, namely Dunaliella salina, Euglena sanguinea and a Nostoc strain.The strains were screened for carotenoid composition with HPLC-UV-APCI-MS with comparison with authentic standards. Carotenoids were purified with open column chromatography, and characterized by LC-UV-MS and NMR.All three species contained a high amount of carotenoids. The composition of the carotenoid pattern was somewhat different compared to literature data. The Dunaliella carotenoid fraction contained lutein (52.1{\%} of total carotenoid), β-carotene (13.1{\%}), violaxanthin (11.5{\%}) and neoxanthin (6.2{\%}) as chief compounds. The chief carotenoids in the Nostoc strain was echinenone (34.9{\%}), while the main components of the Euglena isolate were diatoxanthin (39.0{\%}), lutein (23.7{\%}), an unidentified carotenoid (9.6{\%}) and β-carotene (5.4{\%}). Diatoxanthin is identified by NMR spectroscopy.The carotenoid patterns of the examined strains are somewhat different from the patterns described in the literature. This can be the results of genetic or environmental differences, or combinations thereof. Despite these differences, our study shows the potency of these algae in production of carotenoids, and possibly, usage as food additives.",
keywords = "Carotenoids, HPLC, HPLC-MS, Microalgae",
author = "J. Deli and S. Gonda and Nagy, {L. Z S} and I. Szab{\'o} and G. Guly{\'a}s-Fekete and A. Ag{\'o}cs and K. Marton and G. Vasas",
year = "2014",
month = "11",
day = "1",
doi = "10.1016/j.foodres.2014.05.020",
language = "English",
volume = "65",
pages = "215--223",
journal = "Food Research International",
issn = "0963-9969",
publisher = "Elsevier Limited",
number = "PB",

}

TY - JOUR

T1 - Carotenoid composition of three bloom-forming algae species

AU - Deli, J.

AU - Gonda, S.

AU - Nagy, L. Z S

AU - Szabó, I.

AU - Gulyás-Fekete, G.

AU - Agócs, A.

AU - Marton, K.

AU - Vasas, G.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Algal mass productions are widespread events throughout the world. Most research deals with the environmental impact, ecology and toxicity of these phenomena, but the algae are also promising sources of bioactive natural products, and also are potential food additives. In the current study, we aimed to characterize the carotenoid composition of three non-toxic algal isolates with distinct taxonomical position, namely Dunaliella salina, Euglena sanguinea and a Nostoc strain.The strains were screened for carotenoid composition with HPLC-UV-APCI-MS with comparison with authentic standards. Carotenoids were purified with open column chromatography, and characterized by LC-UV-MS and NMR.All three species contained a high amount of carotenoids. The composition of the carotenoid pattern was somewhat different compared to literature data. The Dunaliella carotenoid fraction contained lutein (52.1% of total carotenoid), β-carotene (13.1%), violaxanthin (11.5%) and neoxanthin (6.2%) as chief compounds. The chief carotenoids in the Nostoc strain was echinenone (34.9%), while the main components of the Euglena isolate were diatoxanthin (39.0%), lutein (23.7%), an unidentified carotenoid (9.6%) and β-carotene (5.4%). Diatoxanthin is identified by NMR spectroscopy.The carotenoid patterns of the examined strains are somewhat different from the patterns described in the literature. This can be the results of genetic or environmental differences, or combinations thereof. Despite these differences, our study shows the potency of these algae in production of carotenoids, and possibly, usage as food additives.

AB - Algal mass productions are widespread events throughout the world. Most research deals with the environmental impact, ecology and toxicity of these phenomena, but the algae are also promising sources of bioactive natural products, and also are potential food additives. In the current study, we aimed to characterize the carotenoid composition of three non-toxic algal isolates with distinct taxonomical position, namely Dunaliella salina, Euglena sanguinea and a Nostoc strain.The strains were screened for carotenoid composition with HPLC-UV-APCI-MS with comparison with authentic standards. Carotenoids were purified with open column chromatography, and characterized by LC-UV-MS and NMR.All three species contained a high amount of carotenoids. The composition of the carotenoid pattern was somewhat different compared to literature data. The Dunaliella carotenoid fraction contained lutein (52.1% of total carotenoid), β-carotene (13.1%), violaxanthin (11.5%) and neoxanthin (6.2%) as chief compounds. The chief carotenoids in the Nostoc strain was echinenone (34.9%), while the main components of the Euglena isolate were diatoxanthin (39.0%), lutein (23.7%), an unidentified carotenoid (9.6%) and β-carotene (5.4%). Diatoxanthin is identified by NMR spectroscopy.The carotenoid patterns of the examined strains are somewhat different from the patterns described in the literature. This can be the results of genetic or environmental differences, or combinations thereof. Despite these differences, our study shows the potency of these algae in production of carotenoids, and possibly, usage as food additives.

KW - Carotenoids

KW - HPLC

KW - HPLC-MS

KW - Microalgae

UR - http://www.scopus.com/inward/record.url?scp=84910000906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910000906&partnerID=8YFLogxK

U2 - 10.1016/j.foodres.2014.05.020

DO - 10.1016/j.foodres.2014.05.020

M3 - Article

AN - SCOPUS:84910000906

VL - 65

SP - 215

EP - 223

JO - Food Research International

JF - Food Research International

SN - 0963-9969

IS - PB

ER -