Cardiovascular responses to catecholamines and interactions with endothelin-1 and adenine nucleosides in the pericardium of the dog heart

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The pericardial fluid may accumulate endogenous regulatory agents, such as catecholamines, endothelin or adenine nucleosides. However, very little information is available on the cardiovascular effects of intrapericardial (i.p.) catecholamines and their interaction with the endogenous endothelins and adenine nucleosides. The cardiovascular effects of increasing doses of i.p.-administered dopamine boluses (0.06-8 μmol/kg, n = 8) were studied in the in situ canine heart: systemic blood pressure, heart rate and left ventricular dP/dt were recorded, and pericardial infusate samples were obtained to measure the changes in endothelin-1 (ET-1), adenosine and inosine levels (enzyme-linked immunosorbent assay and high-performance liquid chromatography methods, respectively). The responses to i.p. dopamine were compared with the effects of i.p. norepinephrine boluses (0.004-0.5 μmol/kg, n = 8). Dopamine elicited dose-dependent increases of heart rate (P <0.01), and the highest dose of dopamine resulted in significant elevation in dP/dt and blood pressure (P <0.01) with a nearly twofold increase of i.p. ET-1 (from 14.3 ± 0.1 pg/mL to 26.1 ± 0.1 pg/mL, P <0.02) and a more than threefold augmentation of i.p. adenosine (from 2.9 ± 0.5 μM to 11.1 ± 3.0 μM, P <0.05), but not of inosine levels. Similar responses were obtained with i.p. norepinephrine. The results confirm that i.p. catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. However, the pattern of catecholamine actions initiated from the pericardium may characteristically differ from that of intravascular ones.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume44
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Pericardium
Endothelin-1
Adenine
Nucleosides
Catecholamines
Dopamine
Dogs
Adenosine
Inosine
Endothelins
Norepinephrine
Heart Rate
Blood Pressure
Canidae
Hemodynamics
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography

Keywords

  • Adenosine
  • Cardiovascular physiology
  • Dopamine
  • Endothelin-1
  • Inosine
  • Norepinephrine
  • Pericardium

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

@article{c7d40273e7c740c380314246271eaeff,
title = "Cardiovascular responses to catecholamines and interactions with endothelin-1 and adenine nucleosides in the pericardium of the dog heart",
abstract = "The pericardial fluid may accumulate endogenous regulatory agents, such as catecholamines, endothelin or adenine nucleosides. However, very little information is available on the cardiovascular effects of intrapericardial (i.p.) catecholamines and their interaction with the endogenous endothelins and adenine nucleosides. The cardiovascular effects of increasing doses of i.p.-administered dopamine boluses (0.06-8 μmol/kg, n = 8) were studied in the in situ canine heart: systemic blood pressure, heart rate and left ventricular dP/dt were recorded, and pericardial infusate samples were obtained to measure the changes in endothelin-1 (ET-1), adenosine and inosine levels (enzyme-linked immunosorbent assay and high-performance liquid chromatography methods, respectively). The responses to i.p. dopamine were compared with the effects of i.p. norepinephrine boluses (0.004-0.5 μmol/kg, n = 8). Dopamine elicited dose-dependent increases of heart rate (P <0.01), and the highest dose of dopamine resulted in significant elevation in dP/dt and blood pressure (P <0.01) with a nearly twofold increase of i.p. ET-1 (from 14.3 ± 0.1 pg/mL to 26.1 ± 0.1 pg/mL, P <0.02) and a more than threefold augmentation of i.p. adenosine (from 2.9 ± 0.5 μM to 11.1 ± 3.0 μM, P <0.05), but not of inosine levels. Similar responses were obtained with i.p. norepinephrine. The results confirm that i.p. catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. However, the pattern of catecholamine actions initiated from the pericardium may characteristically differ from that of intravascular ones.",
keywords = "Adenosine, Cardiovascular physiology, Dopamine, Endothelin-1, Inosine, Norepinephrine, Pericardium",
author = "V. K{\'e}kesi and Ildik{\'o} Toma and Andrea Nagy and M. Rusvai and E. Bar{\'a}t and E. Husz{\'a}r and A. Juh{\'a}sz-Nagy",
year = "2004",
month = "11",
doi = "10.1097/01.fjc.0000166256.12229.d6",
language = "English",
volume = "44",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Cardiovascular responses to catecholamines and interactions with endothelin-1 and adenine nucleosides in the pericardium of the dog heart

AU - Kékesi, V.

AU - Toma, Ildikó

AU - Nagy, Andrea

AU - Rusvai, M.

AU - Barát, E.

AU - Huszár, E.

AU - Juhász-Nagy, A.

PY - 2004/11

Y1 - 2004/11

N2 - The pericardial fluid may accumulate endogenous regulatory agents, such as catecholamines, endothelin or adenine nucleosides. However, very little information is available on the cardiovascular effects of intrapericardial (i.p.) catecholamines and their interaction with the endogenous endothelins and adenine nucleosides. The cardiovascular effects of increasing doses of i.p.-administered dopamine boluses (0.06-8 μmol/kg, n = 8) were studied in the in situ canine heart: systemic blood pressure, heart rate and left ventricular dP/dt were recorded, and pericardial infusate samples were obtained to measure the changes in endothelin-1 (ET-1), adenosine and inosine levels (enzyme-linked immunosorbent assay and high-performance liquid chromatography methods, respectively). The responses to i.p. dopamine were compared with the effects of i.p. norepinephrine boluses (0.004-0.5 μmol/kg, n = 8). Dopamine elicited dose-dependent increases of heart rate (P <0.01), and the highest dose of dopamine resulted in significant elevation in dP/dt and blood pressure (P <0.01) with a nearly twofold increase of i.p. ET-1 (from 14.3 ± 0.1 pg/mL to 26.1 ± 0.1 pg/mL, P <0.02) and a more than threefold augmentation of i.p. adenosine (from 2.9 ± 0.5 μM to 11.1 ± 3.0 μM, P <0.05), but not of inosine levels. Similar responses were obtained with i.p. norepinephrine. The results confirm that i.p. catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. However, the pattern of catecholamine actions initiated from the pericardium may characteristically differ from that of intravascular ones.

AB - The pericardial fluid may accumulate endogenous regulatory agents, such as catecholamines, endothelin or adenine nucleosides. However, very little information is available on the cardiovascular effects of intrapericardial (i.p.) catecholamines and their interaction with the endogenous endothelins and adenine nucleosides. The cardiovascular effects of increasing doses of i.p.-administered dopamine boluses (0.06-8 μmol/kg, n = 8) were studied in the in situ canine heart: systemic blood pressure, heart rate and left ventricular dP/dt were recorded, and pericardial infusate samples were obtained to measure the changes in endothelin-1 (ET-1), adenosine and inosine levels (enzyme-linked immunosorbent assay and high-performance liquid chromatography methods, respectively). The responses to i.p. dopamine were compared with the effects of i.p. norepinephrine boluses (0.004-0.5 μmol/kg, n = 8). Dopamine elicited dose-dependent increases of heart rate (P <0.01), and the highest dose of dopamine resulted in significant elevation in dP/dt and blood pressure (P <0.01) with a nearly twofold increase of i.p. ET-1 (from 14.3 ± 0.1 pg/mL to 26.1 ± 0.1 pg/mL, P <0.02) and a more than threefold augmentation of i.p. adenosine (from 2.9 ± 0.5 μM to 11.1 ± 3.0 μM, P <0.05), but not of inosine levels. Similar responses were obtained with i.p. norepinephrine. The results confirm that i.p. catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. However, the pattern of catecholamine actions initiated from the pericardium may characteristically differ from that of intravascular ones.

KW - Adenosine

KW - Cardiovascular physiology

KW - Dopamine

KW - Endothelin-1

KW - Inosine

KW - Norepinephrine

KW - Pericardium

UR - http://www.scopus.com/inward/record.url?scp=11144290191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144290191&partnerID=8YFLogxK

U2 - 10.1097/01.fjc.0000166256.12229.d6

DO - 10.1097/01.fjc.0000166256.12229.d6

M3 - Article

VL - 44

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

IS - SUPPL. 1

ER -