Cardioprotective mechanisms of Prunus cerasus (sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts

I. Bak, I. Lekli, B. Juhász, Norbert Nagy, E. Varga, J. Váradi, R. Gesztelyi, Gergo Szabo, Levente Szendrei, I. Bácskay, M. Vecsernyés, M. Antal, L. Fésüs, Francois Boucher, Joel De Leiris, A. Tósaki

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Abstract

The effects of kernel extract obtained from sour cherry (Prunus cerasus) seed on the postischemic cardiac recovery were studied in isolated working rat hearts. Rats were treated with various daily doses of the extract for 14 days, and hearts were then isolated and subjected to 30 min of global ischemia followed by 120 min of reperfusion. The incidence of ventricular fibrillation (VF) and tachycardia (VT) fell from their control values of 92% and 100% to 50% (not significant) and 58% (not significant), 17% (P <0.05), and 25% (P <0.05) with the doses of 10 mg/kg and 30 mg/kg of the extract, respectively. Lower concentrations of the extract (1 and 5 mg/kg) failed to significantly reduce the incidence of VF and VT during reperfusion. Sour cherry seed kernel extract (10 and 30 mg/kg) significantly improved the postischemic recovery of cardiac function (coronary flow, aortic flow, and left ventricular developed pressure) during reperfusion. We have also demonstrated that the extract-induced protection in cardiac function significantly reflected in a reduction of infarct size. Immunohistochemistry indicates that a reduction in caspase-3 activity and apoptotic cells by the extract, beside other potential action mechanisms of proanthocyanidin, trans-resveratrol, and flavonoid components of the extract, could be responsible for the cardioprotection in ischemic-reperfused myocardium.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume291
Issue number3
DOIs
Publication statusPublished - 2006

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Reperfusion Injury
Reperfusion
Seeds
Ischemia
Ventricular Fibrillation
Ventricular Tachycardia
Incidence
Recovery of Function
Ventricular Pressure
Cell Extracts
Flavonoids
Caspase 3
Action Potentials
Myocardium
Immunohistochemistry
Prunus avium

Keywords

  • Isolated heart
  • Recovery
  • Sour cherry seed kernel extract

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Cardioprotective mechanisms of Prunus cerasus (sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts",
abstract = "The effects of kernel extract obtained from sour cherry (Prunus cerasus) seed on the postischemic cardiac recovery were studied in isolated working rat hearts. Rats were treated with various daily doses of the extract for 14 days, and hearts were then isolated and subjected to 30 min of global ischemia followed by 120 min of reperfusion. The incidence of ventricular fibrillation (VF) and tachycardia (VT) fell from their control values of 92{\%} and 100{\%} to 50{\%} (not significant) and 58{\%} (not significant), 17{\%} (P <0.05), and 25{\%} (P <0.05) with the doses of 10 mg/kg and 30 mg/kg of the extract, respectively. Lower concentrations of the extract (1 and 5 mg/kg) failed to significantly reduce the incidence of VF and VT during reperfusion. Sour cherry seed kernel extract (10 and 30 mg/kg) significantly improved the postischemic recovery of cardiac function (coronary flow, aortic flow, and left ventricular developed pressure) during reperfusion. We have also demonstrated that the extract-induced protection in cardiac function significantly reflected in a reduction of infarct size. Immunohistochemistry indicates that a reduction in caspase-3 activity and apoptotic cells by the extract, beside other potential action mechanisms of proanthocyanidin, trans-resveratrol, and flavonoid components of the extract, could be responsible for the cardioprotection in ischemic-reperfused myocardium.",
keywords = "Isolated heart, Recovery, Sour cherry seed kernel extract",
author = "I. Bak and I. Lekli and B. Juh{\'a}sz and Norbert Nagy and E. Varga and J. V{\'a}radi and R. Gesztelyi and Gergo Szabo and Levente Szendrei and I. B{\'a}cskay and M. Vecserny{\'e}s and M. Antal and L. F{\'e}s{\"u}s and Francois Boucher and {De Leiris}, Joel and A. T{\'o}saki",
year = "2006",
doi = "10.1152/ajpheart.01243.2005",
language = "English",
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journal = "American Journal of Physiology",
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T1 - Cardioprotective mechanisms of Prunus cerasus (sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts

AU - Bak, I.

AU - Lekli, I.

AU - Juhász, B.

AU - Nagy, Norbert

AU - Varga, E.

AU - Váradi, J.

AU - Gesztelyi, R.

AU - Szabo, Gergo

AU - Szendrei, Levente

AU - Bácskay, I.

AU - Vecsernyés, M.

AU - Antal, M.

AU - Fésüs, L.

AU - Boucher, Francois

AU - De Leiris, Joel

AU - Tósaki, A.

PY - 2006

Y1 - 2006

N2 - The effects of kernel extract obtained from sour cherry (Prunus cerasus) seed on the postischemic cardiac recovery were studied in isolated working rat hearts. Rats were treated with various daily doses of the extract for 14 days, and hearts were then isolated and subjected to 30 min of global ischemia followed by 120 min of reperfusion. The incidence of ventricular fibrillation (VF) and tachycardia (VT) fell from their control values of 92% and 100% to 50% (not significant) and 58% (not significant), 17% (P <0.05), and 25% (P <0.05) with the doses of 10 mg/kg and 30 mg/kg of the extract, respectively. Lower concentrations of the extract (1 and 5 mg/kg) failed to significantly reduce the incidence of VF and VT during reperfusion. Sour cherry seed kernel extract (10 and 30 mg/kg) significantly improved the postischemic recovery of cardiac function (coronary flow, aortic flow, and left ventricular developed pressure) during reperfusion. We have also demonstrated that the extract-induced protection in cardiac function significantly reflected in a reduction of infarct size. Immunohistochemistry indicates that a reduction in caspase-3 activity and apoptotic cells by the extract, beside other potential action mechanisms of proanthocyanidin, trans-resveratrol, and flavonoid components of the extract, could be responsible for the cardioprotection in ischemic-reperfused myocardium.

AB - The effects of kernel extract obtained from sour cherry (Prunus cerasus) seed on the postischemic cardiac recovery were studied in isolated working rat hearts. Rats were treated with various daily doses of the extract for 14 days, and hearts were then isolated and subjected to 30 min of global ischemia followed by 120 min of reperfusion. The incidence of ventricular fibrillation (VF) and tachycardia (VT) fell from their control values of 92% and 100% to 50% (not significant) and 58% (not significant), 17% (P <0.05), and 25% (P <0.05) with the doses of 10 mg/kg and 30 mg/kg of the extract, respectively. Lower concentrations of the extract (1 and 5 mg/kg) failed to significantly reduce the incidence of VF and VT during reperfusion. Sour cherry seed kernel extract (10 and 30 mg/kg) significantly improved the postischemic recovery of cardiac function (coronary flow, aortic flow, and left ventricular developed pressure) during reperfusion. We have also demonstrated that the extract-induced protection in cardiac function significantly reflected in a reduction of infarct size. Immunohistochemistry indicates that a reduction in caspase-3 activity and apoptotic cells by the extract, beside other potential action mechanisms of proanthocyanidin, trans-resveratrol, and flavonoid components of the extract, could be responsible for the cardioprotection in ischemic-reperfused myocardium.

KW - Isolated heart

KW - Recovery

KW - Sour cherry seed kernel extract

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U2 - 10.1152/ajpheart.01243.2005

DO - 10.1152/ajpheart.01243.2005

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VL - 291

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6119

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