Cardioprotection with grapes

Jianhua Cui, B. Juhász, A. Tósaki, Nilanjana Maulik, Dipak K. Das

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Epidemiologic studies suggest that mild-to-moderate wine consumption is associated with a reduced incidence of mortality and morbidity from coronary heart disease. Because wines are produced from grapes, this study was done to determine whether the grapes were equally cardioprotective. Sprague-Dawley male rats were given (orally) standardized grape extract (SGE) (obtained from the California Table Grape Commission, Fresno, CA, U.S.A.) (50, 100, or 200 mg/kg body weight per day) for 3 weeks. Time-matched control experiments were performed by feeding the animals 45 μg/100 g of glucose plus 45 μg/100 g of fructose per day for 3 weeks. After 21 days, rats were killed and the hearts excised and perfused via working mode. Hearts were made ischemic for 30 min followed by 2 h of reperfusion. At 100 mg/kg and at 200 mg/kg, grapes provided significant cardioprotection as evidenced by improved postischemic ventricular recovery (aortic flow, developed pressure, the maximum first derivative of the developed pressure) and reduced amount of myocardial infarction. There were no differences in results between the two groups (100 mg/kg versus 200 mg/kg). No cardioprotection was apparent when rats were given grape samples at a dose of 50 mg/100 g/d. SGE reduced the malonaldehyde content of the heart, indicating reduction of oxidative stress during ischemia and reperfusion. In vitro studies demonstrated that the SGE could directly scavenge superoxide and hydroxyl radicals that are formed in the ischemic reperfused myocardium. The results demonstrate that the hearts of the rats fed SGE are resistant to myocardial ischemia reperfusion injury, suggesting a cardioprotective role of grapes.

Original languageEnglish
Pages (from-to)762-769
Number of pages8
JournalJournal of Cardiovascular Pharmacology
Volume40
Issue number5
DOIs
Publication statusPublished - Nov 1 2002

Fingerprint

Vitis
Wine
Reperfusion
Myocardial Reperfusion Injury
Pressure
Fructose
Reperfusion Injury
Malondialdehyde
Superoxides
Hydroxyl Radical
Myocardial Ischemia
Coronary Disease
Sprague Dawley Rats
Epidemiologic Studies
Myocardium
Oxidative Stress
Ischemia
Myocardial Infarction
Body Weight
Morbidity

Keywords

  • Anti-oxidants
  • Free radicals
  • Grapes
  • Heart
  • Ischemia/reperfusion
  • Polyphenols

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Cardioprotection with grapes. / Cui, Jianhua; Juhász, B.; Tósaki, A.; Maulik, Nilanjana; Das, Dipak K.

In: Journal of Cardiovascular Pharmacology, Vol. 40, No. 5, 01.11.2002, p. 762-769.

Research output: Contribution to journalArticle

Cui, Jianhua ; Juhász, B. ; Tósaki, A. ; Maulik, Nilanjana ; Das, Dipak K. / Cardioprotection with grapes. In: Journal of Cardiovascular Pharmacology. 2002 ; Vol. 40, No. 5. pp. 762-769.
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