Cardiac ultrastructural effects of the platelet-activating factor and its antagonist BN 52021

V. Kecskeméti, I. Balogh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Platelet-activating factor-induced ultrastructural changes of myocardium were examined in isolated perfused guineapig heart. The platelet-activating factor (10-9-10+7 M) caused the following electron microscopic changes: dilated capillaries filled with platelets and aggregated platelets. The endothelial cells adjoining the platelets remained uninjured but pericapillary oedema was observed. in the myocardium intracellular oedema, myofibrillar alterations, decrease of matrix density and rupture of crest in mitochondria can be seen. Ca2+ deposits in the cytoplasm increased and appeared in mitochondria, too. the intramitochondrially localised cytochromoxydase and succinic dehydrogenase activities were decreased. using lanthanum tracer permeability alterations were observed. Pretreatment with BN 52021 (10-6 M) completely prevented the morphological effects of the platelet-activating factor. From these results we conclude that the platelet-activating factor-induced vascular and ischemic like cellular damage appears to play an important role in the pathophysiology of myocardial ischemia.

Original languageEnglish
Pages (from-to)463-470
Number of pages8
JournalExperimental and Toxicologic Pathology
Volume47
Issue number6
DOIs
Publication statusPublished - Jan 1 1995

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Keywords

  • BN 52021, antagonist of platelet activating factor
  • Cardiac effects, platelet activating factor Platelet activating factor antagonist
  • Cardiac ultrastructure
  • Heart, platelet activating factor
  • Platelet activating factor
  • SR
  • T-tubules
  • Z-disc
  • cap
  • capillary
  • ic
  • ic
  • ic
  • intercalated disc
  • intramitochondrial inclusion, floccular
  • intramitochondrial inclusion, intercristal
  • m
  • mitochondrium
  • n
  • nucleus
  • sarcolemma
  • sarcoplasmic reticulumt
  • sl
  • t
  • thc
  • thrombocyte
  • z

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology

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