Carbon source utilisation and evaluation of the Biolog system in the identification of Actinobacillus pleuropneumoniae

L. Makrai, Rita Sárközi, L. Fodor

Research output: Contribution to journalArticle

Abstract

Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. A total of 41 isolates were identified as A. pleuropneumoniae bio-type I and 27 strains as biotype II based on cultural, morphological and biochemical characteristics. The aim of this study was to evaluate metabolic fingerprinting in the species-level identification of A. pleuropneumoniae isolates. Utilisation of carbon sources by these field isolates and six reference strains was characterised by the Biolog system (GN2 Microplate, MicroLog3 Version 4.20.05 software). Twenty-nine field strains were correctly identified by the Biolog system as A. pleuropneumoniae, 36 strains as A. lignieresii, two strains as H. paraphrohaemolyticus and one strain as A. equuli after 24 h of incubation. Among the six A. pleuropneumoniae reference strains the Biolog system identified one strain as A. pleuropneumoniae, four as A. lignieresii and one as H. paraphrohaemolyticus. There was no correlation between biotypes and serotypes of A. pleuropneumoniae and the carbon source utilisation pattern and species identification by the Biolog system. our data indicate that the efficacy of the Biolog system used here could be improved by including phenotypes of more A. pleuropneumoniae strains representing a wider geographical occurrence into the database.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalActa veterinaria Hungarica
Volume67
Issue number3
DOIs
Publication statusPublished - Sep 1 2019

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Actinobacillus pleuropneumoniae
Carbon
carbon
biotypes
Pleuropneumonia
Hungary
Swine
Software
Databases
Phenotype
serotypes

Keywords

  • Actinobacillus pleuropneumoniae
  • Biolog system
  • carbon source utilisation
  • identification

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Carbon source utilisation and evaluation of the Biolog system in the identification of Actinobacillus pleuropneumoniae. / Makrai, L.; Sárközi, Rita; Fodor, L.

In: Acta veterinaria Hungarica, Vol. 67, No. 3, 01.09.2019, p. 327-337.

Research output: Contribution to journalArticle

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