Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers

Peter Boldog, Kata Hajdu, Melinda Magyar, É. Hideg, K. Hernádi, Endre Horváth, Arnaud Magrez, Krisztina Nagy, G. Váró, Laszlo Forró, Laszlo Nagy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Photosensitizers may convert molecular oxygen into reactive oxygen species (ROS) including, e.g., singlet oxygen (1O2), superoxide anion (O2-•), and hydroxyl radicals (OH), chemicals with extremely high cyto- and potential genotoxicity. Photodynamic ROS reactions are determinative in medical photodynamic therapy (cancer treatment with externally added photosensitizers) and in reactions damaging the photosynthetic apparatus of plants (via internal pigments). The primary events of photosynthesis take place in the chlorophyll containing reaction center protein complex (RC), where the energy of light is converted into chemical potential. 1O2 is formed by both bacterial bacteriochlorophylls and plant RC triplet chlorophylls in high light and if the quenching of 1O2 is impaired. In plant physiology, reducing the formation of the ROS and thus lessening photooxidative membrane damage (including the RC protein) and increasing the efficiency of the photochemical energy conversion is of special interest. Carbon nanotubes, in artificial systems, are also known to react with singlet oxygen. To investigate the possibility of 1O2 quenching by carbon nanotubes in a biological system, we studied the effect of carbon nanotubes on 1O2 photogenerated by photosynthetic RCs purified from purple bacteria. 1,3-Diphenylisobenzofuran (DPBF), a dye responding to oxidation by 1O2 with absorption decrease at 420nm was used to measure 1O2 concentrations. 1O2 was produced either from a photosensitizer (methylene blue) or from triplet photosynthetic RCs and the antioxidant capacity of carbon nanotubes was assessed. Less 1O2 was detected by DPBF in the presence of carbon nanotubes, suggesting that these are potential quenchers of this ROS.

Original languageEnglish
Pages (from-to)2539-2543
Number of pages5
JournalPhysica Status Solidi (B) Basic Research
Volume250
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Photosynthetic Reaction Center Complex Proteins
Singlet Oxygen
Carbon Nanotubes
Carbon nanotubes
carbon nanotubes
Photosensitizing Agents
Reactive Oxygen Species
Oxygen
Photosensitizers
oxygen
Chlorophyll
Quenching
chlorophylls
Bacteriochlorophylls
Photodynamic therapy
Oncology
plant physiology
Photosynthesis
Molecular oxygen
Methylene Blue

Keywords

  • Carbon nanotube
  • Reaction center protein
  • Singlet oxygen

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers. / Boldog, Peter; Hajdu, Kata; Magyar, Melinda; Hideg, É.; Hernádi, K.; Horváth, Endre; Magrez, Arnaud; Nagy, Krisztina; Váró, G.; Forró, Laszlo; Nagy, Laszlo.

In: Physica Status Solidi (B) Basic Research, Vol. 250, No. 12, 12.2013, p. 2539-2543.

Research output: Contribution to journalArticle

Boldog, Peter ; Hajdu, Kata ; Magyar, Melinda ; Hideg, É. ; Hernádi, K. ; Horváth, Endre ; Magrez, Arnaud ; Nagy, Krisztina ; Váró, G. ; Forró, Laszlo ; Nagy, Laszlo. / Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers. In: Physica Status Solidi (B) Basic Research. 2013 ; Vol. 250, No. 12. pp. 2539-2543.
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AB - Photosensitizers may convert molecular oxygen into reactive oxygen species (ROS) including, e.g., singlet oxygen (1O2), superoxide anion (O2-•), and hydroxyl radicals (•OH), chemicals with extremely high cyto- and potential genotoxicity. Photodynamic ROS reactions are determinative in medical photodynamic therapy (cancer treatment with externally added photosensitizers) and in reactions damaging the photosynthetic apparatus of plants (via internal pigments). The primary events of photosynthesis take place in the chlorophyll containing reaction center protein complex (RC), where the energy of light is converted into chemical potential. 1O2 is formed by both bacterial bacteriochlorophylls and plant RC triplet chlorophylls in high light and if the quenching of 1O2 is impaired. In plant physiology, reducing the formation of the ROS and thus lessening photooxidative membrane damage (including the RC protein) and increasing the efficiency of the photochemical energy conversion is of special interest. Carbon nanotubes, in artificial systems, are also known to react with singlet oxygen. To investigate the possibility of 1O2 quenching by carbon nanotubes in a biological system, we studied the effect of carbon nanotubes on 1O2 photogenerated by photosynthetic RCs purified from purple bacteria. 1,3-Diphenylisobenzofuran (DPBF), a dye responding to oxidation by 1O2 with absorption decrease at 420nm was used to measure 1O2 concentrations. 1O2 was produced either from a photosensitizer (methylene blue) or from triplet photosynthetic RCs and the antioxidant capacity of carbon nanotubes was assessed. Less 1O2 was detected by DPBF in the presence of carbon nanotubes, suggesting that these are potential quenchers of this ROS.

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