Carbon nanotubes exert basic excitatory enhancement in rat brain slices

Petra Varró, Imola Szigyártó, A. Gergely, E. Kálmán, I. Világi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Carbon nanotubes are promising new tools in biomedicine but they may have yet some unknown influences on the organism. In the present study, the acute effect of solubilized, multi-walled carbon nanotubes (MWCNTs) on basic neuronal functions was examined. Rat brain slices were treated in vitro with nanotube-containing colloid solutions at concentrations of 100-800 μg/ml and evoked field potentials were recorded from the somatosensory cortex and hippocampus. Basic excitability of the treated slices was characterized by the amplitude of field excitatory postsynaptic potentials (fEPSPs) and population spikes. Experimental results indicated significantly higher excitability of treated samples than that of controls. Multiple components in evoked potentials were observed, which is in accordance with the increased excitability of investigated brain areas. Tests of short- and long-term plasticity were also performed, which revealed no difference between control and treated slices. Experimental results suggest an interaction between nanotubes and brain tissue. MWCNTs seem to act on the basic membrane potential of neurons by changing membrane properties or via a mechanism linked to voltage-gated ion channels, rather than influencing specific synaptic transmission. Further investigation is needed to clarify the nature of interactions between nanotubes and brain tissue.

Original languageEnglish
Pages (from-to)137-151
Number of pages15
JournalActa Biologica Hungarica
Volume64
Issue number2
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Carbon Nanotubes
Nanotubes
brain
Rats
Brain
Evoked Potentials
Tissue
membrane
Membranes
Somatosensory Cortex
Excitatory Postsynaptic Potentials
Bioelectric potentials
potential field
Colloids
colloid
Ion Channels
Synaptic Transmission
Membrane Potentials
Neurons
Plasticity

Keywords

  • Carbon nanotubes
  • field potentials
  • hippocampus
  • neocortex
  • rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Carbon nanotubes exert basic excitatory enhancement in rat brain slices. / Varró, Petra; Szigyártó, Imola; Gergely, A.; Kálmán, E.; Világi, I.

In: Acta Biologica Hungarica, Vol. 64, No. 2, 01.06.2013, p. 137-151.

Research output: Contribution to journalArticle

Varró, Petra ; Szigyártó, Imola ; Gergely, A. ; Kálmán, E. ; Világi, I. / Carbon nanotubes exert basic excitatory enhancement in rat brain slices. In: Acta Biologica Hungarica. 2013 ; Vol. 64, No. 2. pp. 137-151.
@article{58089c0dceb74451be326793fdb03f98,
title = "Carbon nanotubes exert basic excitatory enhancement in rat brain slices",
abstract = "Carbon nanotubes are promising new tools in biomedicine but they may have yet some unknown influences on the organism. In the present study, the acute effect of solubilized, multi-walled carbon nanotubes (MWCNTs) on basic neuronal functions was examined. Rat brain slices were treated in vitro with nanotube-containing colloid solutions at concentrations of 100-800 μg/ml and evoked field potentials were recorded from the somatosensory cortex and hippocampus. Basic excitability of the treated slices was characterized by the amplitude of field excitatory postsynaptic potentials (fEPSPs) and population spikes. Experimental results indicated significantly higher excitability of treated samples than that of controls. Multiple components in evoked potentials were observed, which is in accordance with the increased excitability of investigated brain areas. Tests of short- and long-term plasticity were also performed, which revealed no difference between control and treated slices. Experimental results suggest an interaction between nanotubes and brain tissue. MWCNTs seem to act on the basic membrane potential of neurons by changing membrane properties or via a mechanism linked to voltage-gated ion channels, rather than influencing specific synaptic transmission. Further investigation is needed to clarify the nature of interactions between nanotubes and brain tissue.",
keywords = "Carbon nanotubes, field potentials, hippocampus, neocortex, rat",
author = "Petra Varr{\'o} and Imola Szigy{\'a}rt{\'o} and A. Gergely and E. K{\'a}lm{\'a}n and I. Vil{\'a}gi",
year = "2013",
month = "6",
day = "1",
doi = "10.1556/ABiol.64.2013.2.1",
language = "English",
volume = "64",
pages = "137--151",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Carbon nanotubes exert basic excitatory enhancement in rat brain slices

AU - Varró, Petra

AU - Szigyártó, Imola

AU - Gergely, A.

AU - Kálmán, E.

AU - Világi, I.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Carbon nanotubes are promising new tools in biomedicine but they may have yet some unknown influences on the organism. In the present study, the acute effect of solubilized, multi-walled carbon nanotubes (MWCNTs) on basic neuronal functions was examined. Rat brain slices were treated in vitro with nanotube-containing colloid solutions at concentrations of 100-800 μg/ml and evoked field potentials were recorded from the somatosensory cortex and hippocampus. Basic excitability of the treated slices was characterized by the amplitude of field excitatory postsynaptic potentials (fEPSPs) and population spikes. Experimental results indicated significantly higher excitability of treated samples than that of controls. Multiple components in evoked potentials were observed, which is in accordance with the increased excitability of investigated brain areas. Tests of short- and long-term plasticity were also performed, which revealed no difference between control and treated slices. Experimental results suggest an interaction between nanotubes and brain tissue. MWCNTs seem to act on the basic membrane potential of neurons by changing membrane properties or via a mechanism linked to voltage-gated ion channels, rather than influencing specific synaptic transmission. Further investigation is needed to clarify the nature of interactions between nanotubes and brain tissue.

AB - Carbon nanotubes are promising new tools in biomedicine but they may have yet some unknown influences on the organism. In the present study, the acute effect of solubilized, multi-walled carbon nanotubes (MWCNTs) on basic neuronal functions was examined. Rat brain slices were treated in vitro with nanotube-containing colloid solutions at concentrations of 100-800 μg/ml and evoked field potentials were recorded from the somatosensory cortex and hippocampus. Basic excitability of the treated slices was characterized by the amplitude of field excitatory postsynaptic potentials (fEPSPs) and population spikes. Experimental results indicated significantly higher excitability of treated samples than that of controls. Multiple components in evoked potentials were observed, which is in accordance with the increased excitability of investigated brain areas. Tests of short- and long-term plasticity were also performed, which revealed no difference between control and treated slices. Experimental results suggest an interaction between nanotubes and brain tissue. MWCNTs seem to act on the basic membrane potential of neurons by changing membrane properties or via a mechanism linked to voltage-gated ion channels, rather than influencing specific synaptic transmission. Further investigation is needed to clarify the nature of interactions between nanotubes and brain tissue.

KW - Carbon nanotubes

KW - field potentials

KW - hippocampus

KW - neocortex

KW - rat

UR - http://www.scopus.com/inward/record.url?scp=84878810675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878810675&partnerID=8YFLogxK

U2 - 10.1556/ABiol.64.2013.2.1

DO - 10.1556/ABiol.64.2013.2.1

M3 - Article

VL - 64

SP - 137

EP - 151

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2

ER -