Capsaicin-sensitive mechanisms in the modulation of rat colonic vascular permeability under physiological and pathological conditions

Z. Szepes, J. Kiss, T. Molnár, D. Lamarque, G. Jancsó, F. László

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Inflammatory boewel disease (IBD) causes a prolonged life-quality reduction of patients and high costs for health services. The aim of this study was to explore the possible involvement of peptidergic capsaicin-sensitive afferent nerves (CSN) in the pathogenesis of IBD. For the defunctionalization of colonic CSN, the lower part of the colon (1-4 cm from the anus) was exposed through a midline laparotomy and small pieces of gelfoam moistened with a solution of capsaicin (1%, 100 μL) was applied onto the serosal surface for 30 min in male Wistar rats. Colonic vascular permeability was assessed by measuring the extravasation of [125I] human serum albumin (2 μCi/kg, iv, 2 h prior to killing). Two months after capsaicin treatment a significant increase in albumin extravasation was found in the lower (P < 0.005), but not in the upper (5-8 cm from the anus) part of the colon as compared to the sham-operated control. Intrarectal 18 cm from anus) administration of trinitrobenzene-sulphonic acid (TNBS; 30 mg/rat) induced similar plasma leakage in the lower and upper colon of control (CSN-intact) rats (P < 0.001) 1 week later. TNBS + ethanol (50%) produced further extravasation throughout the colon (P < 0.001) of CSN-intact animals. In the lower colon of capsaicin-pretreated rats TNBS-alone provoked an increase in plasma extravasation (P < 0.001) similar to that caused by TNBS + ethanol in CSN-intact rats. In the upper colon there was no difference in the effect of TNBS-alone on plasma leakage between control (CSN-intact) and CSN-depleted rats. The results suggest that capsaicin-sensitive nerves may play a significant protective/anti-inflammatory role in the colon under normal and pathological conditions.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalJournal of Physiology Paris
Volume91
Issue number3-5
DOIs
Publication statusPublished - Jan 1 1997

Keywords

  • Capsaicin-sensitivie innervation
  • Inflammatory bowel disease
  • Sensory neuropeptide
  • Vascular endothelium
  • Vascular permeability

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

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