Capillary gel electrophoretic separation of DNA restriction fragments in a discontinuous buffer system

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Abstract

Separation of single and double stranded DNA molecules by capillary gel electrophoresis has a rapidly growing interest. Similar to the polyacrylamide slab gel based separation methods, in capillary gel electrophoresis, the peak/band spacing usually decreases with the increasing size/length of the DNA molecule. Additionally, employing the regularly used Tris-borate buffer system, fronting peaks are often obtained. By the application of an electrolyte step gradient during capillary gel electrophoretic separation of dsDNA molecules, the apparent peak shape can be improved and the required analysis time decreased.

Original languageEnglish
Pages (from-to)321-324
Number of pages4
JournalJournal of Chromatography A
Volume744
Issue number1-2
DOIs
Publication statusPublished - Sep 13 1996

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Keywords

  • Buffer composition
  • Capillary gel electrophoresis
  • DNA

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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