Capillary gel affinity electrophoresis of DNA fragments

A. Guttman, Nelson Cooke

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The incorporation of an affinity ligand within a polyacrylamide gel provides a general means of manipulating the selectivity of capillary gel electrophoresis separations. As an example of this approach, high resolution of DNA restriction fragments by capillary gel affinity electrophoresis has been achieved by adding a soluble intercalating agent, ethidium bromide, to the gel-buffer system. A migration model has been developed that can be used for selectivity optimization. Various parameters, such as ligand concentration and applied electric field, have been examined in terms of their influence on retention and selectivity of different-size DNA molecules. From this study, high-resolution separations have been developed with efficiencies as high as 107 theoretical plates per meter.

Original languageEnglish
Pages (from-to)2038-2042
Number of pages5
JournalAnalytical Chemistry
Volume63
Issue number18
Publication statusPublished - Sep 15 1991

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Electrophoresis
Gels
DNA
Intercalating Agents
Ligands
Ethidium
Buffers
Electric fields
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Capillary gel affinity electrophoresis of DNA fragments. / Guttman, A.; Cooke, Nelson.

In: Analytical Chemistry, Vol. 63, No. 18, 15.09.1991, p. 2038-2042.

Research output: Contribution to journalArticle

Guttman, A. ; Cooke, Nelson. / Capillary gel affinity electrophoresis of DNA fragments. In: Analytical Chemistry. 1991 ; Vol. 63, No. 18. pp. 2038-2042.
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