Capillary electrophoretic analysis of wild type and mutant Proteus penneri outer membrane proteins

Ildikó Kustos, Vilmos Tóth, Béla Kocsis, Ildikó Kerepesi, Levente Emody, Ferenc Kilár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study the virulence factors, outer membrane proteins (OMP), lipopolysaccharides (LPS), hemolysin, and the in vivo and in vitro virulence of wild-type Proteus penned 357 and its two isogenic mutant variants - a transposon and a spontaneous mutant - were examined. The OMPs of these variants were analyzed by a new and fast technique, 'dynamic sieving' capillary electrophoresis (CE). The OMP profiles were dominated by two peaks (39 and 43 kDa). In the P. penneri clone examined, both the transposon and the spontaneous mutations induced significant changes in the OMP patterns (in the relative percentage of the dominant proteins). CE was suitable for the comparative analysis of bacterial protein patterns in the genetic variants of this strain, and provided valuable results in connection with the bacteriological virulence. The LPS composition of the genetic variants also showed alterations. The wild type of P. penneri 357 showed a typical ladder pattern, an 'S' form, and the mutants possessed 'R' LPS patterns (only few bands) in the gels. In the bacteriological virulence tests the wild type of P. penneri 357 was virulent in the in vivo, and toxic in the in vitro assays, while both mutants showed neither toxicity nor pathogenicity.

Original languageEnglish
Pages (from-to)3020-3027
Number of pages8
JournalELECTROPHORESIS
Volume21
Issue number14
DOIs
Publication statusPublished - Sep 28 2000

Keywords

  • Bacterial virulence
  • Capillary electrophoresis
  • Outer membrane proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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