Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids

Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, A. Guttman, Jose Carlos Diez-Masa, Mercedes De Frutos

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages207-225
Number of pages19
Volume984
ISBN (Print)9781627032957
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume984
ISSN (Print)10643745

Fingerprint

Infant Food
Orosomucoid
Lactoglobulins
Capillary Electrophoresis
Allergens
Protein Isoforms
Lasers
Biomarkers
Fluorescence
Food
Serum
Proteins

Keywords

  • Alpha 1-acid glycoprotein
  • Biomarker
  • Capillary electrophoresis
  • Detection
  • Isoform
  • Lactoglobulin
  • LIF
  • Milk
  • Orosomucoid
  • Sample preparation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Garrido-Medina, R., Puerta, A., Pelaez-Lorenzo, C., Rivera-Monroy, Z., Guttman, A., Diez-Masa, J. C., & De Frutos, M. (2013). Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids. In Methods in Molecular Biology (Vol. 984, pp. 207-225). (Methods in Molecular Biology; Vol. 984). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-296-4_15

Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices : Food and biological fluids. / Garrido-Medina, Raul; Puerta, Angel; Pelaez-Lorenzo, Cristina; Rivera-Monroy, Zuly; Guttman, A.; Diez-Masa, Jose Carlos; De Frutos, Mercedes.

Methods in Molecular Biology. Vol. 984 Humana Press Inc., 2013. p. 207-225 (Methods in Molecular Biology; Vol. 984).

Research output: Chapter in Book/Report/Conference proceedingChapter

Garrido-Medina, R, Puerta, A, Pelaez-Lorenzo, C, Rivera-Monroy, Z, Guttman, A, Diez-Masa, JC & De Frutos, M 2013, Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids. in Methods in Molecular Biology. vol. 984, Methods in Molecular Biology, vol. 984, Humana Press Inc., pp. 207-225. https://doi.org/10.1007/978-1-62703-296-4_15
Garrido-Medina R, Puerta A, Pelaez-Lorenzo C, Rivera-Monroy Z, Guttman A, Diez-Masa JC et al. Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids. In Methods in Molecular Biology. Vol. 984. Humana Press Inc. 2013. p. 207-225. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-296-4_15
Garrido-Medina, Raul ; Puerta, Angel ; Pelaez-Lorenzo, Cristina ; Rivera-Monroy, Zuly ; Guttman, A. ; Diez-Masa, Jose Carlos ; De Frutos, Mercedes. / Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices : Food and biological fluids. Methods in Molecular Biology. Vol. 984 Humana Press Inc., 2013. pp. 207-225 (Methods in Molecular Biology).
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