Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids

Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa, Mercedes De Frutos

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.

Original languageEnglish
Title of host publicationCapillary Electrophoresis of Biomolecules
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages207-225
Number of pages19
ISBN (Print)9781627032957
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume984
ISSN (Print)1064-3745

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Keywords

  • Alpha 1-acid glycoprotein
  • Biomarker
  • Capillary electrophoresis
  • Detection
  • Isoform
  • LIF
  • Lactoglobulin
  • Milk
  • Orosomucoid
  • Sample preparation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Garrido-Medina, R., Puerta, A., Pelaez-Lorenzo, C., Rivera-Monroy, Z., Guttman, A., Diez-Masa, J. C., & De Frutos, M. (2013). Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices: Food and biological fluids. In Capillary Electrophoresis of Biomolecules: Methods and Protocols (pp. 207-225). (Methods in Molecular Biology; Vol. 984). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-296-4_15