Capillary electrophoresis study of outer membrane proteins of Pseudomonas strains upon antibiotic treatment

Tamás Kustos, Ildikó Kustos, Edit Gonda, Béla Kocsis, György Szabó, F. Kilár

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Nosocomial wound infections by Pseudomonas aeruginosa strains have increasing importance in orthopaedic surgery. Outer membrane protein composition and cell-surface hydrophobicity of the bacteria have strong influence on adhesion to living tissues or artificial medical devices. Outer membrane proteins of five Pseudomonas strains (KT 2, KT 7, KT 25, KT 28, KT 39) isolated from orthopaedic patients' wounds and one standard strain NIH Hungary 170000 isolated from pus were examined. The capillary electrophoretic patterns of the outer membrane proteins were characteristic to each bacterial strains possessing different relative ratios of major and minor proteins. Antibiotic treatment of bacteria with three antibiotics, cefotaximum, amoxicillinum/clavulinic acid and amikacinum (applied often in prophylaxis and treatment of patients) induced changes in the electrophoretic profiles showing that outer membrane protein composition was altered significantly. The most pronounced alterations in the electrophoretic patterns after antibiotic treatment were obtained in the cases of the strains KT 2, KT 7 and KT 28. The amikacinum administration strongly decreased the relative percentage of the 38800 rel. mol. mass protein in KT 2 (from 20 to 6%), while the relative amount of the same protein increased significantly in KT 7 and KT 28 after cefotaximum treatment (from 2 to 16% and from 12 to 28%, respectively). Decrease in cell-surface hydrophobicity was also observed by salt aggregation test. The results obtained can be used to determine the therapeutic concentrations of antibiotics to induce changes in the adhesion properties of bacteria.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalJournal of Chromatography A
Volume979
Issue number1-2
DOIs
Publication statusPublished - Dec 6 2002

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Pseudomonas
Membrane Proteins
Anti-Bacterial Agents
Bacteria
Orthopedics
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Adhesion
Therapeutics
Proteins
Suppuration
Hungary
Wound Infection
Cross Infection
Chemical analysis
Pseudomonas aeruginosa
Surgery
Agglomeration

Keywords

  • Antibiotics
  • Proteins
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Capillary electrophoresis study of outer membrane proteins of Pseudomonas strains upon antibiotic treatment. / Kustos, Tamás; Kustos, Ildikó; Gonda, Edit; Kocsis, Béla; Szabó, György; Kilár, F.

In: Journal of Chromatography A, Vol. 979, No. 1-2, 06.12.2002, p. 277-284.

Research output: Contribution to journalArticle

Kustos, Tamás ; Kustos, Ildikó ; Gonda, Edit ; Kocsis, Béla ; Szabó, György ; Kilár, F. / Capillary electrophoresis study of outer membrane proteins of Pseudomonas strains upon antibiotic treatment. In: Journal of Chromatography A. 2002 ; Vol. 979, No. 1-2. pp. 277-284.
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