Capillary electrophoresis separation of vinpocetine and related compounds: Prediction of electrophoretic mobilities in partly aqueous media

Károly Mazák, Zoltán Szakács, Andreás Nemes, Béla Noszál

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Offord's equation, a relationship between electrophoretic mobility and charge, size and shape of peptides, has been extended to quantitate the electrophoretic mobility of vinca alkaloids. Partly aqueous protonation constants and the derived theoretical mobilities have been proven to be able to predict experimental electrophoretic mobilities. In practice, seven vincamine derivatives of very low water-solubility were separated by capillary electrophoresis. Buffer total concentration, apparent pH and methanol content, the three most important parameters of the running buffer, were used in triangular resolution mapping to characterize separation. Even though electrophoresis is well known to slow down in partly aqueous media, under our optimized circumstances a baseline separation was achieved within 8 min in each case.

Original languageEnglish
Pages (from-to)2417-2423
Number of pages7
JournalELECTROPHORESIS
Volume21
Issue number12
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Capillary electrophoresis
  • Mobility prediction
  • Resolution mapping
  • Vinca alkaloids
  • Vinpocetine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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