Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In N-glycosylation analysis of biopharmaceuticals, analytical need depends on the phase of the manufacturing process. All important glycoanalysis steps are thoroughly discussed. Carbohydrate sequencing by exoglycosidase arrays is described in conjunction with capillary electrophoresis (CE) to identify linkage and positional isomers. A possible automated workflow for N-glycosylation based on CE is outlined.

Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalTrAC - Trends in Analytical Chemistry
Volume48
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Glycosylation
Capillary electrophoresis
electrokinesis
Glycoside Hydrolases
Isomers
carbohydrate
manufacturing
Carbohydrates
analysis
need
isomer

Keywords

  • Automated workflow
  • Biopharmaceuticals
  • Biotherapeutics
  • Capillary electrophoresis (CE)
  • Carbohydrate sequencing
  • Glycoanalysis
  • High-sensitivity detection
  • Immunogenic epitope
  • N-glycosylation
  • Purity assessment

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals. / Guttman, A.

In: TrAC - Trends in Analytical Chemistry, Vol. 48, 07.2013, p. 132-143.

Research output: Contribution to journalArticle

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