Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

In N-glycosylation analysis of biopharmaceuticals, analytical need depends on the phase of the manufacturing process. All important glycoanalysis steps are thoroughly discussed. Carbohydrate sequencing by exoglycosidase arrays is described in conjunction with capillary electrophoresis (CE) to identify linkage and positional isomers. A possible automated workflow for N-glycosylation based on CE is outlined.

Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalTrAC - Trends in Analytical Chemistry
Volume48
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Automated workflow
  • Biopharmaceuticals
  • Biotherapeutics
  • Capillary electrophoresis (CE)
  • Carbohydrate sequencing
  • Glycoanalysis
  • High-sensitivity detection
  • Immunogenic epitope
  • N-glycosylation
  • Purity assessment

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Fingerprint Dive into the research topics of 'Capillary electrophoresis in the N-glycosylation analysis of biopharmaceuticals'. Together they form a unique fingerprint.

  • Cite this