Capillary electrophoresis chips for screening of endotoxin chemotypes from whole-cell lysates

Anikó Kilár, Zoltán Péterfi, Eszter Csorba, Ferenc Kilár, Béla Kocsis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A fast microchip electrophoresis method was developed to analyze and differentiate bacterial endotoxins directly from whole-cell lysates after removal of the proteinaceous components with proteinase K digestion and a precipitation of the endotoxin components. The partially purified endotoxin components were visualized by the interaction with dodecyl sulphate and then a fluorescent dye. The lipopolysaccharide (LPS) profiles can be directly evaluated from digested bacterial cells, and the electrophoresis patterns very closely resembled to those of pure LPSs, and the R and S chemotypes can be used to assign the strains. The method has been found to be useful in the screening of a large number of bacterial mutants and the structural characterization of endotoxins extracted only from 1 ml cultures.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Chromatography A
Volume1206
Issue number1
DOIs
Publication statusPublished - Oct 3 2008

Keywords

  • Bacterial mutants
  • Endotoxin screening
  • LPS
  • Microchip electrophoresis
  • Whole-cell lysate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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