Capillary chromatography/microelectrospray mass spectrometry used for the identification of putative cyclin-dependent kinase inhibitory protein in medicago

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20 Citations (Scopus)

Abstract

A home-built capillary chromatography/microelectrospray system was used for peptide mass mapping of a putative cyclin-dependent kinase inhibitor protein with molecular mass of 8.5 kDa. The masses of identified tryptic fragments were then input to a protein database search routine. Daughter ion scans were done only on the most abundant tryptic fragments. On the basis of protein database search results and tandem mass spectrometric measurements the bioactive protein was identified as ubiquitin.

Original languageEnglish
Pages (from-to)1564-1568
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume12
Issue number20
DOIs
Publication statusPublished - 1998

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Chromatography
Mass spectrometry
Cyclin-Dependent Kinase Inhibitor Proteins
Proteins
Molecular mass
Ubiquitin
Ions
Peptides
Cyclin-Dependent Kinases

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

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abstract = "A home-built capillary chromatography/microelectrospray system was used for peptide mass mapping of a putative cyclin-dependent kinase inhibitor protein with molecular mass of 8.5 kDa. The masses of identified tryptic fragments were then input to a protein database search routine. Daughter ion scans were done only on the most abundant tryptic fragments. On the basis of protein database search results and tandem mass spectrometric measurements the bioactive protein was identified as ubiquitin.",
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AU - Kele, Z.

AU - Janáky, T.

AU - Mészáros, T.

AU - Fehér, A.

AU - Dudits, D.

AU - Szabó, P.

PY - 1998

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