Cannabinoids inhibit the release of [3H]glutamate from rodent hippocampal synaptosomes via a novel CB1 receptor-independent action

Attila Köfalvi, E. Vízi, Catherine Ledent, B. Sperlágh

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

In this study we investigated the effect of cannabinoids on [ 3H]glutamate release from hippocampal synaptosomes of rat and CB 1-null mutant mouse. In the rat, cannabinoid receptor agonists, i.e. CP55,940 (EC50, 0.84 μM), WIN55,212-2 (EC50, 3.47 μM), ACEA (EC50, 17.8 μM), and R-(+)-methanandamide (EC 50, 19.8 μM) concentration-dependently inhibited the 25-mM-K + depolarization-evoked release of [3H]glutamate and, among them, WIN55,212-2 displayed the greatest efficacy. The CB1 receptor antagonists SR141716A (1-5 μM) and AM251 (1 μM) and the VR 1 vanilloid receptor antagonist capsazepine (10 μM) did not antagonize the effect of the agonists. SR141716A by itself attenuated the evoked [3H]glutamate release. WIN55,212-2 inhibited the release of [3H]glutamate in CB1-/- mice as well. These data demonstrate that the action of cannabinoids on glutamate release in the hippocampus is pharmacologically distinct and independent from the cloned CB1 receptor.

Original languageEnglish
Pages (from-to)1973-1978
Number of pages6
JournalEuropean Journal of Neuroscience
Volume18
Issue number7
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Cannabinoid Receptor CB1
Cannabinoids
Synaptosomes
rimonabant
Glutamic Acid
Rodentia
Cannabinoid Receptor Agonists
Hippocampus

Keywords

  • CB receptor
  • Hippocampus
  • Knockout
  • SR141716A
  • WIN55,212-2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cannabinoids inhibit the release of [3H]glutamate from rodent hippocampal synaptosomes via a novel CB1 receptor-independent action. / Köfalvi, Attila; Vízi, E.; Ledent, Catherine; Sperlágh, B.

In: European Journal of Neuroscience, Vol. 18, No. 7, 10.2003, p. 1973-1978.

Research output: Contribution to journalArticle

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