Cannabinoid receptor binding did not vary in several hypothalamic nuclei after hypothalamic deafferentation

J. Romero, T. Wenger, R. De Miguel, J. A. Ramos, J. J. Fernández-Ruiz

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Cannabinoid receptors are sparsely distributed in the hypothalamic nuclei, although they seem to be located on key neurons because their activation produces important neuroendocrine effects. In the present study, we have examined whether cannabinoid receptor-containing hypothalamic neurons are intrinsic or extrinsic to this brain region. To this end, we have examined whether the hypothalamic deafferentation was followed by a general loss of cannabinoid receptor binding, thus suggesting that cannabinoid receptor-containing neurons would have their cell bodies outside the hypothalamus, or whether this was followed by no changes in binding, thus suggesting that cannabinoid receptors would be located on intrinsic neurons. Three experimental groups were then analyzed: (i) animals with complete hypothalamic deafferentation in both sides; (ii) hemideafferentated animals; and (iii) sham-operated animals. In the three cases, cannabinoid receptor binding did not vary among these three groups in any of the hypothalamic nuclei analyzed. These were the arcuate nucleus, ventromedial hypothalamic nucleus, lateral and dorsal hypothalamic areas, paraventricular nucleus and medial preoptic area. This clearly supports the view that cannabinoid receptor-containing neurons into the hypothalamus are all intrinsic to this brain region.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalLife Sciences
Volume63
Issue number5
DOIs
Publication statusPublished - Jun 1998

Fingerprint

Cannabinoid Receptors
Neurons
Animals
Hypothalamus
Brain
Ventromedial Hypothalamic Nucleus
Lateral Hypothalamic Area
Arcuate Nucleus of Hypothalamus
Preoptic Area
Paraventricular Hypothalamic Nucleus
Chemical activation
Cells

Keywords

  • Cannabinoid receptors
  • Cannabinoids
  • Hypothalamic deafferentation
  • Hypothalamic nuclei
  • Hypothalamus

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cannabinoid receptor binding did not vary in several hypothalamic nuclei after hypothalamic deafferentation. / Romero, J.; Wenger, T.; De Miguel, R.; Ramos, J. A.; Fernández-Ruiz, J. J.

In: Life Sciences, Vol. 63, No. 5, 06.1998, p. 351-356.

Research output: Contribution to journalArticle

Romero, J. ; Wenger, T. ; De Miguel, R. ; Ramos, J. A. ; Fernández-Ruiz, J. J. / Cannabinoid receptor binding did not vary in several hypothalamic nuclei after hypothalamic deafferentation. In: Life Sciences. 1998 ; Vol. 63, No. 5. pp. 351-356.
@article{2df9dff9dbf8441ab985449155f4ea2a,
title = "Cannabinoid receptor binding did not vary in several hypothalamic nuclei after hypothalamic deafferentation",
abstract = "Cannabinoid receptors are sparsely distributed in the hypothalamic nuclei, although they seem to be located on key neurons because their activation produces important neuroendocrine effects. In the present study, we have examined whether cannabinoid receptor-containing hypothalamic neurons are intrinsic or extrinsic to this brain region. To this end, we have examined whether the hypothalamic deafferentation was followed by a general loss of cannabinoid receptor binding, thus suggesting that cannabinoid receptor-containing neurons would have their cell bodies outside the hypothalamus, or whether this was followed by no changes in binding, thus suggesting that cannabinoid receptors would be located on intrinsic neurons. Three experimental groups were then analyzed: (i) animals with complete hypothalamic deafferentation in both sides; (ii) hemideafferentated animals; and (iii) sham-operated animals. In the three cases, cannabinoid receptor binding did not vary among these three groups in any of the hypothalamic nuclei analyzed. These were the arcuate nucleus, ventromedial hypothalamic nucleus, lateral and dorsal hypothalamic areas, paraventricular nucleus and medial preoptic area. This clearly supports the view that cannabinoid receptor-containing neurons into the hypothalamus are all intrinsic to this brain region.",
keywords = "Cannabinoid receptors, Cannabinoids, Hypothalamic deafferentation, Hypothalamic nuclei, Hypothalamus",
author = "J. Romero and T. Wenger and {De Miguel}, R. and Ramos, {J. A.} and Fern{\'a}ndez-Ruiz, {J. J.}",
year = "1998",
month = "6",
doi = "10.1016/S0024-3205(98)00283-5",
language = "English",
volume = "63",
pages = "351--356",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Cannabinoid receptor binding did not vary in several hypothalamic nuclei after hypothalamic deafferentation

AU - Romero, J.

AU - Wenger, T.

AU - De Miguel, R.

AU - Ramos, J. A.

AU - Fernández-Ruiz, J. J.

PY - 1998/6

Y1 - 1998/6

N2 - Cannabinoid receptors are sparsely distributed in the hypothalamic nuclei, although they seem to be located on key neurons because their activation produces important neuroendocrine effects. In the present study, we have examined whether cannabinoid receptor-containing hypothalamic neurons are intrinsic or extrinsic to this brain region. To this end, we have examined whether the hypothalamic deafferentation was followed by a general loss of cannabinoid receptor binding, thus suggesting that cannabinoid receptor-containing neurons would have their cell bodies outside the hypothalamus, or whether this was followed by no changes in binding, thus suggesting that cannabinoid receptors would be located on intrinsic neurons. Three experimental groups were then analyzed: (i) animals with complete hypothalamic deafferentation in both sides; (ii) hemideafferentated animals; and (iii) sham-operated animals. In the three cases, cannabinoid receptor binding did not vary among these three groups in any of the hypothalamic nuclei analyzed. These were the arcuate nucleus, ventromedial hypothalamic nucleus, lateral and dorsal hypothalamic areas, paraventricular nucleus and medial preoptic area. This clearly supports the view that cannabinoid receptor-containing neurons into the hypothalamus are all intrinsic to this brain region.

AB - Cannabinoid receptors are sparsely distributed in the hypothalamic nuclei, although they seem to be located on key neurons because their activation produces important neuroendocrine effects. In the present study, we have examined whether cannabinoid receptor-containing hypothalamic neurons are intrinsic or extrinsic to this brain region. To this end, we have examined whether the hypothalamic deafferentation was followed by a general loss of cannabinoid receptor binding, thus suggesting that cannabinoid receptor-containing neurons would have their cell bodies outside the hypothalamus, or whether this was followed by no changes in binding, thus suggesting that cannabinoid receptors would be located on intrinsic neurons. Three experimental groups were then analyzed: (i) animals with complete hypothalamic deafferentation in both sides; (ii) hemideafferentated animals; and (iii) sham-operated animals. In the three cases, cannabinoid receptor binding did not vary among these three groups in any of the hypothalamic nuclei analyzed. These were the arcuate nucleus, ventromedial hypothalamic nucleus, lateral and dorsal hypothalamic areas, paraventricular nucleus and medial preoptic area. This clearly supports the view that cannabinoid receptor-containing neurons into the hypothalamus are all intrinsic to this brain region.

KW - Cannabinoid receptors

KW - Cannabinoids

KW - Hypothalamic deafferentation

KW - Hypothalamic nuclei

KW - Hypothalamus

UR - http://www.scopus.com/inward/record.url?scp=17744403167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17744403167&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(98)00283-5

DO - 10.1016/S0024-3205(98)00283-5

M3 - Article

C2 - 9714422

AN - SCOPUS:17744403167

VL - 63

SP - 351

EP - 356

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 5

ER -