Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary

Sándor Szekeres, Elena Claudia Coipan, Krisztina Rigó, Gábor Majoros, Setareh Jahfari, Hein Sprong, G. Földvári

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalTicks and Tick-borne Diseases
Volume6
Issue number2
DOIs
Publication statusPublished - Mar 1 2015

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Anaplasma phagocytophilum
Hungary
Ticks
ticks
Rodentia
Apodemus flavicollis
rodents
skin (animal)
Apodemus agrarius
Murinae
Arvicolinae
Skin
spleen
sampling
Spleen
Tick Infestations
Microtus arvalis
tick infestations
Clethrionomys glareolus
Multiplex Polymerase Chain Reaction

Keywords

  • Anaplasma phagocytophilum
  • Candidatus neoehrlichia mikurensis
  • Hungary
  • Prevalence
  • Rodent
  • Tick

ASJC Scopus subject areas

  • Infectious Diseases
  • Insect Science
  • Parasitology
  • Microbiology

Cite this

Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary. / Szekeres, Sándor; Claudia Coipan, Elena; Rigó, Krisztina; Majoros, Gábor; Jahfari, Setareh; Sprong, Hein; Földvári, G.

In: Ticks and Tick-borne Diseases, Vol. 6, No. 2, 01.03.2015, p. 111-116.

Research output: Contribution to journalArticle

Szekeres, Sándor ; Claudia Coipan, Elena ; Rigó, Krisztina ; Majoros, Gábor ; Jahfari, Setareh ; Sprong, Hein ; Földvári, G. / Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary. In: Ticks and Tick-borne Diseases. 2015 ; Vol. 6, No. 2. pp. 111-116.
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