Candidate plant gene homologues in grapevine involved in Agrobacterium transformation

Tamás Deák, Tünde Kupi, Róbert Oláh, L. Lakatos, L. Kemény, György D. Bisztray, E. Szegedi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The grapevine (Vitis vinifera) genome was analyzed in silico for homologues of plant genes involved in Agrobacterium transformation in Arabidopsis thaliana and Nicotiana spp. Grapevine homologues of the glucomannan 4-betamannosyltransferase 9 gene CslA-09 involved in bacterial attachment to the cell wall, homologues of reticulon-like proteins BTI1, 2, 3 and RAB8 GTPases, both involved in T-DNA transfer to the host cell, homologues of VirE2 interacting protein VIP1 that contributes to the targeting of T-DNA into the nucleus and to its integration, and homologues of the histone protein H2A, which promotes the expression of T-DNA encoded genes, were selected. Sequences homologous to the arabinogalactan-protein AtAGP17 were not found in the grape genome. Seventeen selected candidates were tested by semiquantitative RT-PCR analysis for changes in their expression levels upon inoculation with Agrobacterium tumefaciens C58. Of the tested homologues, the expression of VvRab8a, VvVip1a and two histone genes (VvHta2 and VvHta10) increased significantly, therefore we hypothesize that these might be involved in Agrobacterium transformation of V. vinifera.

Original languageEnglish
Pages (from-to)1001-1009
Number of pages9
JournalCentral European Journal of Biology
Volume8
Issue number10
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Plant Genes
Agrobacterium
Vitis
Genes
Histones
histones
Vitis vinifera
Genome
Agrobacterium tumefaciens
Proteins
genes
GTP Phosphohydrolases
Sequence Homology
Arabidopsis
transfer DNA
arabinogalactan proteins
Computer Simulation
Cell Wall
Tobacco
genome

Keywords

  • Crown gall disease
  • Pathogen-induced gene expression
  • Semiquantitative PCR
  • Vitis vinifera

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Candidate plant gene homologues in grapevine involved in Agrobacterium transformation. / Deák, Tamás; Kupi, Tünde; Oláh, Róbert; Lakatos, L.; Kemény, L.; Bisztray, György D.; Szegedi, E.

In: Central European Journal of Biology, Vol. 8, No. 10, 11.2013, p. 1001-1009.

Research output: Contribution to journalArticle

Deák, Tamás ; Kupi, Tünde ; Oláh, Róbert ; Lakatos, L. ; Kemény, L. ; Bisztray, György D. ; Szegedi, E. / Candidate plant gene homologues in grapevine involved in Agrobacterium transformation. In: Central European Journal of Biology. 2013 ; Vol. 8, No. 10. pp. 1001-1009.
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