Candidate genes of cuticle formation show characteristic expression in the fruit skin of apple

Zsolt Albert, Balázs Ivanics, Attila Molnár, András Miskó, Magdolna Tóth, I. Papp

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Based on the Arabidopsis model of production and transport of cuticular components in silico analysis of apple EST and genomic sequences identified candidate genes potentially involved in these processes. Expression profiling of the selected genes in apple leaves and fruit tissues at the stage of full tree ripeness showed them to be active in fruit skin, in some cases in a tissue-specific manner. Apart from sequence conservation, characteristic transcript profiles make these genes likely participants of fruit cuticle formation. Genes with putative functions in the fatty acid elongase complex, wax and cutin modifications, transport and a potential regulator were identified this way. Activity of some of the putative genes can be correlated with the known wax composition of the 'Florina' cultivar. Year-to-year and cultivar specific variations in expression of some of these genes indicate plasticity of lipid biosynthetic pathways in apple. Coordinated expression of several cuticle associated genes further supports their proposed role in cuticular lipid metabolism. The presented data suggest conservation of some key determinants of biosynthesis and transport of cuticular lipids between Arabidopsis epidermis and apple fruit skin.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalPlant Growth Regulation
Volume70
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Malus
Fruit
apples
Skin
fruits
Waxes
Genes
genes
Arabidopsis
waxes
Lipids
Biosynthetic Pathways
Expressed Sequence Tags
Gene Expression Profiling
Lipid Metabolism
Epidermis
Computer Simulation
cutin
cultivars
epidermis (plant)

Keywords

  • Cuticle
  • Cutin
  • Epidermis
  • Gene expression
  • Malus domestica
  • Wax

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Physiology

Cite this

Candidate genes of cuticle formation show characteristic expression in the fruit skin of apple. / Albert, Zsolt; Ivanics, Balázs; Molnár, Attila; Miskó, András; Tóth, Magdolna; Papp, I.

In: Plant Growth Regulation, Vol. 70, No. 1, 2013, p. 71-78.

Research output: Contribution to journalArticle

Albert, Zsolt ; Ivanics, Balázs ; Molnár, Attila ; Miskó, András ; Tóth, Magdolna ; Papp, I. / Candidate genes of cuticle formation show characteristic expression in the fruit skin of apple. In: Plant Growth Regulation. 2013 ; Vol. 70, No. 1. pp. 71-78.
@article{059d99cff2e649b9bc983f1dfc50fefa,
title = "Candidate genes of cuticle formation show characteristic expression in the fruit skin of apple",
abstract = "Based on the Arabidopsis model of production and transport of cuticular components in silico analysis of apple EST and genomic sequences identified candidate genes potentially involved in these processes. Expression profiling of the selected genes in apple leaves and fruit tissues at the stage of full tree ripeness showed them to be active in fruit skin, in some cases in a tissue-specific manner. Apart from sequence conservation, characteristic transcript profiles make these genes likely participants of fruit cuticle formation. Genes with putative functions in the fatty acid elongase complex, wax and cutin modifications, transport and a potential regulator were identified this way. Activity of some of the putative genes can be correlated with the known wax composition of the 'Florina' cultivar. Year-to-year and cultivar specific variations in expression of some of these genes indicate plasticity of lipid biosynthetic pathways in apple. Coordinated expression of several cuticle associated genes further supports their proposed role in cuticular lipid metabolism. The presented data suggest conservation of some key determinants of biosynthesis and transport of cuticular lipids between Arabidopsis epidermis and apple fruit skin.",
keywords = "Cuticle, Cutin, Epidermis, Gene expression, Malus domestica, Wax",
author = "Zsolt Albert and Bal{\'a}zs Ivanics and Attila Moln{\'a}r and Andr{\'a}s Misk{\'o} and Magdolna T{\'o}th and I. Papp",
year = "2013",
doi = "10.1007/s10725-012-9779-y",
language = "English",
volume = "70",
pages = "71--78",
journal = "Plant Growth Regulation",
issn = "0167-6903",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Candidate genes of cuticle formation show characteristic expression in the fruit skin of apple

AU - Albert, Zsolt

AU - Ivanics, Balázs

AU - Molnár, Attila

AU - Miskó, András

AU - Tóth, Magdolna

AU - Papp, I.

PY - 2013

Y1 - 2013

N2 - Based on the Arabidopsis model of production and transport of cuticular components in silico analysis of apple EST and genomic sequences identified candidate genes potentially involved in these processes. Expression profiling of the selected genes in apple leaves and fruit tissues at the stage of full tree ripeness showed them to be active in fruit skin, in some cases in a tissue-specific manner. Apart from sequence conservation, characteristic transcript profiles make these genes likely participants of fruit cuticle formation. Genes with putative functions in the fatty acid elongase complex, wax and cutin modifications, transport and a potential regulator were identified this way. Activity of some of the putative genes can be correlated with the known wax composition of the 'Florina' cultivar. Year-to-year and cultivar specific variations in expression of some of these genes indicate plasticity of lipid biosynthetic pathways in apple. Coordinated expression of several cuticle associated genes further supports their proposed role in cuticular lipid metabolism. The presented data suggest conservation of some key determinants of biosynthesis and transport of cuticular lipids between Arabidopsis epidermis and apple fruit skin.

AB - Based on the Arabidopsis model of production and transport of cuticular components in silico analysis of apple EST and genomic sequences identified candidate genes potentially involved in these processes. Expression profiling of the selected genes in apple leaves and fruit tissues at the stage of full tree ripeness showed them to be active in fruit skin, in some cases in a tissue-specific manner. Apart from sequence conservation, characteristic transcript profiles make these genes likely participants of fruit cuticle formation. Genes with putative functions in the fatty acid elongase complex, wax and cutin modifications, transport and a potential regulator were identified this way. Activity of some of the putative genes can be correlated with the known wax composition of the 'Florina' cultivar. Year-to-year and cultivar specific variations in expression of some of these genes indicate plasticity of lipid biosynthetic pathways in apple. Coordinated expression of several cuticle associated genes further supports their proposed role in cuticular lipid metabolism. The presented data suggest conservation of some key determinants of biosynthesis and transport of cuticular lipids between Arabidopsis epidermis and apple fruit skin.

KW - Cuticle

KW - Cutin

KW - Epidermis

KW - Gene expression

KW - Malus domestica

KW - Wax

UR - http://www.scopus.com/inward/record.url?scp=84876429942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876429942&partnerID=8YFLogxK

U2 - 10.1007/s10725-012-9779-y

DO - 10.1007/s10725-012-9779-y

M3 - Article

AN - SCOPUS:84876429942

VL - 70

SP - 71

EP - 78

JO - Plant Growth Regulation

JF - Plant Growth Regulation

SN - 0167-6903

IS - 1

ER -