cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF synthesis in mouse hepatocytes

J. Mandl, I. Muchá, G. Bánhegyi, György Mészáros, A. Faragó, Zoltán Spolarics, R. Machovich, Ferenc Antoni, Tamás Garzó

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The role of cAMP dependent regulation in thromboxane A2, prostacyclin and PGF synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N6,O2-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA2, PGI2 and PGF by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.

Original languageEnglish
Pages (from-to)761-772
Number of pages12
JournalProstaglandins
Volume36
Issue number6
DOIs
Publication statusPublished - 1988

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Thromboxane A2
Dinoprost
Epoprostenol
Cyclic AMP-Dependent Protein Kinases
Hepatocytes
Calcium Ionophores
Calcimycin
Prostaglandins
Radioimmunoassay
Membranes
Catalytic Domain
Phosphorylation
Hot Temperature
Adenosine Triphosphate
Cell Membrane
Liver
Derivatives
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF synthesis in mouse hepatocytes. / Mandl, J.; Muchá, I.; Bánhegyi, G.; Mészáros, György; Faragó, A.; Spolarics, Zoltán; Machovich, R.; Antoni, Ferenc; Garzó, Tamás.

In: Prostaglandins, Vol. 36, No. 6, 1988, p. 761-772.

Research output: Contribution to journalArticle

Mandl, J. ; Muchá, I. ; Bánhegyi, G. ; Mészáros, György ; Faragó, A. ; Spolarics, Zoltán ; Machovich, R. ; Antoni, Ferenc ; Garzó, Tamás. / cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF synthesis in mouse hepatocytes. In: Prostaglandins. 1988 ; Vol. 36, No. 6. pp. 761-772.
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AU - Muchá, I.

AU - Bánhegyi, G.

AU - Mészáros, György

AU - Faragó, A.

AU - Spolarics, Zoltán

AU - Machovich, R.

AU - Antoni, Ferenc

AU - Garzó, Tamás

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