Camostate- and caerulein-induced delay of gastric empyting in the rat: Effect of CCK receptor antagonists

G. Varga, Carmelo Scarpignato

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of camostate, a potent releaser of endogenous cholecystokinin (CCK), and of caerulein, an amphibian peptide mimicking the biological actions of CCK, as well as of selective CCK receptor antagonists on gastric emptying of liquids was studied in the rat. Oral administration of camostate (200 mg/kg with the liquid test meal preceded by the same dose 10 min before the meal) significantly delayed gastric emptying of saline, an effect which was completely blocked by previous administration of the CCK(A) receptor antagonist, devazepide, at a dose (1 mg/kg i.v.) unable to modify the emptying rate when administered alone. Caerulein (0.03-30 nmol/kg i.v.) also delayed the emptying rate in a dose-dependent manner, with an ID50 of 3.94 nmol/kg. The effect of the peptide was also inhibited by devazepide. The CCK(B) receptor antagonist, L365,260 (3 R-(+)-N-(2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepine-3-y l)N'-(3-methylphenyl)-urea; 3 mg/kg i.v.), was completely unable to modify the CCK (both endogenous and exogenous)-induced delay in gastric emptying. Repeated (7 days) camostate administration did not modify the gastric motor response to endogenous CCK, thus, suggesting that adaptation did not take place. These results demonstrate that endogenous and exogenous CCK delays gastric emptying of liquids through stimulation of CCK(A) receptors and suggest that adaptation of the gastric motor response to CCK does not occur.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalEuropean Journal of Pharmacology
Volume306
Issue number1-3
DOIs
Publication statusPublished - Jun 13 1996

Fingerprint

Cholecystokinin Receptors
Ceruletide
Cholecystokinin
Stomach
Gastric Emptying
Devazepide
Cholecystokinin A Receptor
Meals
Cholecystokinin B Receptor
Peptides
Amphibians
Oral Administration
camostat

Keywords

  • caerulein
  • camostate
  • CCK (cholecystokinin)
  • CCK receptor antagonist
  • gastric emptying

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Camostate- and caerulein-induced delay of gastric empyting in the rat : Effect of CCK receptor antagonists. / Varga, G.; Scarpignato, Carmelo.

In: European Journal of Pharmacology, Vol. 306, No. 1-3, 13.06.1996, p. 153-159.

Research output: Contribution to journalArticle

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