Callus initiation and plant regeneration from inflorescence primordia of the intergeneric hybrid Agropyron repens (L.) Beauv.x Bromus inermis Leyss. cv. nanus on a modified nutritive medium

G. Gyulai, J. Janovszky, E. Kiss, L. Lelik, A. Csillag, L. Heszky

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17 Citations (Scopus)

Abstract

Plant regeneration from callus of intergeneric hybrid Agropyron repens (L.) Beauv. x Bromus inermis Leyss cv. nanus (AGROMUS) was carried out on a new culture medium designated medium-F. Within 21 days of the plating of inflorescence primordia the initiated callus showed globular structures. From the 21st day of culture, one step plant regeneration occurred on the callus without subculture. The new basal medium reported in this work was effective in callus initiation and plant regeneration of the hybrid AGROMUS by (i) the reduction of the total ion strength (2.6 g/l, 22.5 mM) of macroelements compared to MS (4.5 g/l,45.2 mM), (ii) the use of NH4NO3 as the sole N-source, and (iii) the application of KH2PO4 at an 8 times higher concentration (1160 mg/l,8.5 mM) when compared to the Murashige and Skoog medium composition. This medium provided a 2 to 10 fold reduction in the 2,4-dichlorophenoxyacetic acid supplement needed for the callus initiation and one step plant regeneration after a gibberellic acid (2 mg/l, for 5 days) pretreatment of tillers. The regenerated plantlets were subcultured in multi-shoot culture and potted in soil to grow for further analysis.

Original languageEnglish
Pages (from-to)266-269
Number of pages4
JournalPlant Cell Reports
Volume11
Issue number5-6
DOIs
Publication statusPublished - Jun 1992

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Elymus repens subsp. repens
Bromus inermis
callus
inflorescences
tillers
gibberellic acid
2,4-D
plantlets
pretreatment
culture media
ions
shoots
soil

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Callus initiation and plant regeneration from inflorescence primordia of the intergeneric hybrid Agropyron repens (L.) Beauv.x Bromus inermis Leyss. cv. nanus on a modified nutritive medium",
abstract = "Plant regeneration from callus of intergeneric hybrid Agropyron repens (L.) Beauv. x Bromus inermis Leyss cv. nanus (AGROMUS) was carried out on a new culture medium designated medium-F. Within 21 days of the plating of inflorescence primordia the initiated callus showed globular structures. From the 21st day of culture, one step plant regeneration occurred on the callus without subculture. The new basal medium reported in this work was effective in callus initiation and plant regeneration of the hybrid AGROMUS by (i) the reduction of the total ion strength (2.6 g/l, 22.5 mM) of macroelements compared to MS (4.5 g/l,45.2 mM), (ii) the use of NH4NO3 as the sole N-source, and (iii) the application of KH2PO4 at an 8 times higher concentration (1160 mg/l,8.5 mM) when compared to the Murashige and Skoog medium composition. This medium provided a 2 to 10 fold reduction in the 2,4-dichlorophenoxyacetic acid supplement needed for the callus initiation and one step plant regeneration after a gibberellic acid (2 mg/l, for 5 days) pretreatment of tillers. The regenerated plantlets were subcultured in multi-shoot culture and potted in soil to grow for further analysis.",
author = "G. Gyulai and J. Janovszky and E. Kiss and L. Lelik and A. Csillag and L. Heszky",
year = "1992",
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T1 - Callus initiation and plant regeneration from inflorescence primordia of the intergeneric hybrid Agropyron repens (L.) Beauv.x Bromus inermis Leyss. cv. nanus on a modified nutritive medium

AU - Gyulai, G.

AU - Janovszky, J.

AU - Kiss, E.

AU - Lelik, L.

AU - Csillag, A.

AU - Heszky, L.

PY - 1992/6

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