Callus formation from protoplasts of a sugarbeet cell suspension culture

L. Szabados, Carina Gaggero

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated. The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protoplast division was also considerably stimulated by the addition of 250 mg/l casein hydrolysate, 200 mg/l yeast extract, and 20% v/v conditioned culture medium to the protoplast culture medium. The highest division rate (up to 35% of the protoplasts) was achieved at a density of 4×104- 1×105 protoplasts/ml. From the colonies callus and suspension cultures were readily obtained.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalPlant Cell Reports
Volume4
Issue number4
DOIs
Publication statusPublished - Aug 1985

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callus formation
sugar beet
cell suspension culture
protoplasts
culture media
casein hydrolysates
Beta vulgaris
zeatin
yeast extract
naphthaleneacetic acid
plant hormones
2,4-D
callus

ASJC Scopus subject areas

  • Plant Science

Cite this

Callus formation from protoplasts of a sugarbeet cell suspension culture. / Szabados, L.; Gaggero, Carina.

In: Plant Cell Reports, Vol. 4, No. 4, 08.1985, p. 195-198.

Research output: Contribution to journalArticle

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