Calcium uptake of rat brain synaptosomes as a function of membrane potential under different depolarizing conditions.

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The uptake of 45Ca2+ was measured in brain synaptosomes under conditions designed to depolarize the membranes. Membrane potential was estimated from the distribution of 86Rb+ between the intra‐ and extracellular compartments. K+ depolarization (8‐60 mM) only increased "a2+ uptake beyond a threshold depolarization of about 10 mV, whereas veratridine (5‐40 microM) induced an increased Ca2+ uptake at a concentration which depolarized the membrane less than this threshold. Ouabain did not enhance Ca2+ uptake, but the depolarization it produced did not reach threshold. Ca2+ influx already stimulated by K+ depolarization can be further enhanced by veratridine without any parallel change in membrane potential. Only the pathway mediating Ca2+ uptake during K+ depolarization can be inactivated: Ca2+ uptake evoked by K+ depolarization is decreased in synaptosomes pre‐depolarized in the presence of a high concentration of K+. In contrast, pre‐depolarization does not change Ca2+ uptake evoked by veratridine. Ca2+ channel blockers, such as verapamil and diltiazem but not nifedipine, in concentrations of 10‐100 microM, decrease the stimulation of Ca2+ uptake by high K+ concentration without influencing depolarization, whereas the effect of veratridine on Ca2+ uptake is only inhibited when its effect on Na+ channels is also prevented. It is concluded that Ca2+ uptake during K+ depolarization proceeds through voltage‐dependent Ca2+ channels similar to those of squid axon, whereas veratridine activates an additional Ca2+ entry, possibly via influx through open Na+ channels. Different quantitative relationships are found between acetylcholine release of synaptosomes and the amount of Ca2+ taken up by different mechanisms: the same amount of Ca2+ uptake is accompanied by a greater increase of acetylcholine release if the uptake is induced by K+ depolarization rather than veratridine.

Original languageEnglish
Pages (from-to)363-377
Number of pages15
JournalThe Journal of Physiology
Issue number1
Publication statusPublished - Mar 1 1986

ASJC Scopus subject areas

  • Physiology

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