Calcium released from intracellular stores inhibits GABA(A)-mediated currents in ganglion cells of the turtle retina

Abram Akopian, R. Gábriel, Paul Witkovsky

Research output: Contribution to journalArticle

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Abstract

We studied spiking neurons isolated from turtle retina by the whole cell version of the patch clamp. The studied cells had perikaryal diameters >15 μm and fired multiple spikes in response to depolarizing current steps, indicating they were ganglion cells. In symmetrical [Cl-], currents elicited by puffs of 100 μM γ-aminobutyric acid (GABA) were inward at a holding potential of -80 mV. All of the GABA-evoked current was blocked by SR95331 (20 μM), indicating that it was mediated by a GABA(A) receptor. The GABA- evoked currents were unaltered by eliciting a transmembrane calcium current either just before or during the response to GABA. On the other hand caffeine (10 mM), which induces Ca2+ release from intracellular stores, inhibited the GABA-evoked current on average by 30%. The caffeine effect was blocked by introducing the calcium buffer bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) into the cell but was unaffected by replacing [Ca2+](o) with equimolar cobalt. Thapsigargin (10 μM), an inhibitor of intracellular calcium pumps, and ryanodine (20 μM), which depletes intracellular calcium stores, both markedly reduced a caffeine-induced inhibition of the GABA- evoked current. Another activator of intracellular calcium release, inositol trisphosphate (IP3; 50 μM), also progressively reduced the GABA-induced current when introduced into the cell. Dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP; 0.5 mM), a membrane-permeable analogue of cAMP, did not reduce GABA-evoked currents, suggesting that cAMP-dependent kinases are not involved in suppressing GABA(A) currents, whereas calmidazolium (30 μM) and cyclosporin A (20 μM), which inhibit Ca/calmodulin-dependent phosphatases, did reduce the caffeine-induced inhibition of the GABA-evoked current. Alkaline phosphatase (150 μg/ml) and calcineurin (300 μg/ml) had a similar action to caffeine or IP3. Antibodies directed against the ryanodine receptor or the IP3 receptor reacted with the great majority of neurons in the ganglion cell layer. We found that these two antibodies colocalized in large ganglion cells. In summary, intracellular calcium plays a role in reducing the currents elicited by GABA, acting through GABA(A) receptors. The modulatory action of calcium on GABA responses appears to work through one or more Ca-dependent phosphatases.

Original languageEnglish
Pages (from-to)1105-1115
Number of pages11
JournalJournal of Neurophysiology
Volume80
Issue number3
Publication statusPublished - Sep 1998

Fingerprint

Turtles
Ganglia
gamma-Aminobutyric Acid
Retina
Calcium
Caffeine
GABA-A Receptors
Phosphoric Monoester Hydrolases
calmidazolium
Aminobutyrates
Inositol 1,4,5-Trisphosphate Receptors
Neurons
Ryanodine
Bucladesine
Ryanodine Receptor Calcium Release Channel
Thapsigargin
Antibodies
Calcineurin
Inositol
Calmodulin

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Calcium released from intracellular stores inhibits GABA(A)-mediated currents in ganglion cells of the turtle retina. / Akopian, Abram; Gábriel, R.; Witkovsky, Paul.

In: Journal of Neurophysiology, Vol. 80, No. 3, 09.1998, p. 1105-1115.

Research output: Contribution to journalArticle

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abstract = "We studied spiking neurons isolated from turtle retina by the whole cell version of the patch clamp. The studied cells had perikaryal diameters >15 μm and fired multiple spikes in response to depolarizing current steps, indicating they were ganglion cells. In symmetrical [Cl-], currents elicited by puffs of 100 μM γ-aminobutyric acid (GABA) were inward at a holding potential of -80 mV. All of the GABA-evoked current was blocked by SR95331 (20 μM), indicating that it was mediated by a GABA(A) receptor. The GABA- evoked currents were unaltered by eliciting a transmembrane calcium current either just before or during the response to GABA. On the other hand caffeine (10 mM), which induces Ca2+ release from intracellular stores, inhibited the GABA-evoked current on average by 30{\%}. The caffeine effect was blocked by introducing the calcium buffer bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) into the cell but was unaffected by replacing [Ca2+](o) with equimolar cobalt. Thapsigargin (10 μM), an inhibitor of intracellular calcium pumps, and ryanodine (20 μM), which depletes intracellular calcium stores, both markedly reduced a caffeine-induced inhibition of the GABA- evoked current. Another activator of intracellular calcium release, inositol trisphosphate (IP3; 50 μM), also progressively reduced the GABA-induced current when introduced into the cell. Dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP; 0.5 mM), a membrane-permeable analogue of cAMP, did not reduce GABA-evoked currents, suggesting that cAMP-dependent kinases are not involved in suppressing GABA(A) currents, whereas calmidazolium (30 μM) and cyclosporin A (20 μM), which inhibit Ca/calmodulin-dependent phosphatases, did reduce the caffeine-induced inhibition of the GABA-evoked current. Alkaline phosphatase (150 μg/ml) and calcineurin (300 μg/ml) had a similar action to caffeine or IP3. Antibodies directed against the ryanodine receptor or the IP3 receptor reacted with the great majority of neurons in the ganglion cell layer. We found that these two antibodies colocalized in large ganglion cells. In summary, intracellular calcium plays a role in reducing the currents elicited by GABA, acting through GABA(A) receptors. The modulatory action of calcium on GABA responses appears to work through one or more Ca-dependent phosphatases.",
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N2 - We studied spiking neurons isolated from turtle retina by the whole cell version of the patch clamp. The studied cells had perikaryal diameters >15 μm and fired multiple spikes in response to depolarizing current steps, indicating they were ganglion cells. In symmetrical [Cl-], currents elicited by puffs of 100 μM γ-aminobutyric acid (GABA) were inward at a holding potential of -80 mV. All of the GABA-evoked current was blocked by SR95331 (20 μM), indicating that it was mediated by a GABA(A) receptor. The GABA- evoked currents were unaltered by eliciting a transmembrane calcium current either just before or during the response to GABA. On the other hand caffeine (10 mM), which induces Ca2+ release from intracellular stores, inhibited the GABA-evoked current on average by 30%. The caffeine effect was blocked by introducing the calcium buffer bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) into the cell but was unaffected by replacing [Ca2+](o) with equimolar cobalt. Thapsigargin (10 μM), an inhibitor of intracellular calcium pumps, and ryanodine (20 μM), which depletes intracellular calcium stores, both markedly reduced a caffeine-induced inhibition of the GABA- evoked current. Another activator of intracellular calcium release, inositol trisphosphate (IP3; 50 μM), also progressively reduced the GABA-induced current when introduced into the cell. Dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP; 0.5 mM), a membrane-permeable analogue of cAMP, did not reduce GABA-evoked currents, suggesting that cAMP-dependent kinases are not involved in suppressing GABA(A) currents, whereas calmidazolium (30 μM) and cyclosporin A (20 μM), which inhibit Ca/calmodulin-dependent phosphatases, did reduce the caffeine-induced inhibition of the GABA-evoked current. Alkaline phosphatase (150 μg/ml) and calcineurin (300 μg/ml) had a similar action to caffeine or IP3. Antibodies directed against the ryanodine receptor or the IP3 receptor reacted with the great majority of neurons in the ganglion cell layer. We found that these two antibodies colocalized in large ganglion cells. In summary, intracellular calcium plays a role in reducing the currents elicited by GABA, acting through GABA(A) receptors. The modulatory action of calcium on GABA responses appears to work through one or more Ca-dependent phosphatases.

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