Calcium microdomains in aspiny dendrites

Jesse H. Goldberg, Gabor Tamas, Dmitriy Aronov, Rafael Yuste

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Abstract

Dendritic spines receive excitatory synapses and serve as calcium compartments, which appear to be necessary for input-specific synaptic plasticity. Dendrites of GABAergic interneurons have few or no spines and thus do not possess a clear morphological basis for synapse-specific compartmentalization. We demonstrate using two-photon calcium imaging that activation of single synapses on aspiny dendrites of neocortical fast spiking (FS) interneurons creates highly localized calcium microdomains, often restricted to less than 1 μm of dendritic space. We confirm using ultrastructural reconstruction of imaged dendrites the absence of any morphological basis for this compartmentalization and show that it is dependent on the fast kinetics of calcium-permeable (CP) AMPA receptors and fast local extrusion via the Na+/Ca2+ exchanger. Because aspiny dendrites throughout the CNS express CP-AMPA receptors, we propose that CP-AMPA receptors mediate a spine-free mechanism of input-specific calcium compartmentalization.

Original languageEnglish
Pages (from-to)807-821
Number of pages15
JournalNeuron
Volume40
Issue number4
DOIs
Publication statusPublished - Nov 13 2003

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Goldberg, J. H., Tamas, G., Aronov, D., & Yuste, R. (2003). Calcium microdomains in aspiny dendrites. Neuron, 40(4), 807-821. https://doi.org/10.1016/S0896-6273(03)00714-1