Calcium ion uptake, somatotropin release, and fine structure of somatotrophs in cultures of the rat anterior pituitary upon the action of an oligopeptide (Boc-Gln-Leu-Lysinal)

Gy Rappay, G. Makara, Gy Gaál, V. Garamvölgyi, I. Nagy, S. Bajusz, Gy Széll

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cultured cells from the anterior pituitary glands of adult rats were treated with the tripeptide aldehyde proteinase inhibitor, Boc-Gln-Leu-Lys-H. The oligopeptide had a profound relesing effect on growth hormone, whereas the prolactin release remained unchanged at 10-3 mol/l drug concentration after an incubation for 2 h. In the presence of the oligopeptide a time- and dose-dependent calcium influx into cultured cells has been shown which was proved to be almost completely antagonized with magnesium ions but not with Nifedipine. In addition, radioactive calcium ions could be detected in a number of cells by light microscopic autoradiography when cultures were treated with Boc-Gln-Leu-Lys-H for short periods. The selective Gel action of the oligopeptide on growth hormone producing cells has been demonstrated also in fine structural investigations: multigranular and single exocytotic profiles have been observed. Accordingly, we have postulated that Boc-Gln-Leu-Lysinal mimics the effects of the known ionophores. Its mode of action needs, however, further studies especially on isolated somatotrophs.

Original languageEnglish
Pages (from-to)517-521
Number of pages5
JournalHistochemistry
Volume91
Issue number6
DOIs
Publication statusPublished - Nov 1989

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Somatotrophs
Oligopeptides
Growth Hormone
Ions
Calcium
Cultured Cells
Anterior Pituitary Gland
Ionophores
Nifedipine
Autoradiography
Aldehydes
Prolactin
Magnesium
Peptide Hydrolases
Cell Count
Gels
Light
Pharmaceutical Preparations
butyloxycarbonyl-glutaminyl-leucyl-lysinal

ASJC Scopus subject areas

  • Anatomy

Cite this

Calcium ion uptake, somatotropin release, and fine structure of somatotrophs in cultures of the rat anterior pituitary upon the action of an oligopeptide (Boc-Gln-Leu-Lysinal). / Rappay, Gy; Makara, G.; Gaál, Gy; Garamvölgyi, V.; Nagy, I.; Bajusz, S.; Széll, Gy.

In: Histochemistry, Vol. 91, No. 6, 11.1989, p. 517-521.

Research output: Contribution to journalArticle

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