Calcium homeostasis is modified in skeletal muscle fibers of small ankyrin1 knockout mice

Enrico Pierantozzi, P. Szentesi, Dána Al-Gaadi, Tamás Oláh, Beatrix Dienes, Mónika Sztretye, Daniela Rossi, Vincenzo Sorrentino, L. Csernoch

Research output: Contribution to journalArticle

Abstract

Small Ankyrins (sAnk1) are muscle-specific isoforms generated by the Ank1 gene that participate in the organization of the sarcoplasmic reticulum (SR) of striated muscles. Accordingly, the volume of SR tubules localized around the myofibrils is strongly reduced in skeletal muscle fibers of 4- and 10-month-old sAnk1 knockout (KO) mice, while additional structural alterations only develop with aging. To verify whether the lack of sAnk1 also alters intracellular Ca2+ handling, cytosolic Ca2+ levels were analyzed in stimulated skeletal muscle fibers from 4- and 10-month-old sAnk1 KO mice. The SR Ca2+ content was reduced in sAnk1 KO mice regardless of age. The amplitude of the Ca2+ transients induced by depolarizing pulses was decreased in myofibers of sAnk1 KO with respect to wild type (WT) fibers, while their voltage dependence was not affected. Furthermore, analysis of spontaneous Ca2+ release events (sparks) on saponin-permeabilized muscle fibers indicated that the frequency of sparks was significantly lower in fibers from 4-month-old KO mice compared to WT. Furthermore, both the amplitude and spatial spread of sparks were significantly smaller in muscle fibers from both 4- and 10-month-old KO mice compared to WT. These data suggest that the absence of sAnk1 results in an impairment of SR Ca2+ release, likely as a consequence of a decreased Ca2+ store due to the reduction of the SR volume in sAnk1 KO muscle fibers.

Original languageEnglish
Article number3361
JournalInternational journal of molecular sciences
Volume20
Issue number13
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

sarcoplasmic reticulum
knockout mice
muscle fibers
homeostasis
skeletal muscle
Skeletal Muscle Fibers
Sarcoplasmic Reticulum
Knockout Mice
Muscle
calcium
Calcium
Homeostasis
sparks
Fibers
Muscles
Electric sparks
Ankyrins
fibers
Striated Muscle
Myofibrils

Keywords

  • Calcium release
  • sAnk1
  • Sarcoplasmic reticulum
  • Spark

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Calcium homeostasis is modified in skeletal muscle fibers of small ankyrin1 knockout mice. / Pierantozzi, Enrico; Szentesi, P.; Al-Gaadi, Dána; Oláh, Tamás; Dienes, Beatrix; Sztretye, Mónika; Rossi, Daniela; Sorrentino, Vincenzo; Csernoch, L.

In: International journal of molecular sciences, Vol. 20, No. 13, 3361, 01.07.2019.

Research output: Contribution to journalArticle

Pierantozzi, Enrico ; Szentesi, P. ; Al-Gaadi, Dána ; Oláh, Tamás ; Dienes, Beatrix ; Sztretye, Mónika ; Rossi, Daniela ; Sorrentino, Vincenzo ; Csernoch, L. / Calcium homeostasis is modified in skeletal muscle fibers of small ankyrin1 knockout mice. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 13.
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