Calcium dynamics of cortical astrocytic networks in vivo

Hajime Hirase, Lifen Qian, P. Barthó, György Buzsáki

Research output: Contribution to journalArticle

278 Citations (Scopus)

Abstract

Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca2+]i events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca2+]i events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca 2+]i activity in individual cells and a robust coordination of [Ca2+]i signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.

Original languageEnglish
JournalPLoS Biology
Volume2
Issue number4
DOIs
Publication statusPublished - 2004

Fingerprint

Astrocytes
astrocytes
Calcium
calcium
Brain
brain
Photons
Confocal Microscopy
Neuroglia
Cerebral Cortex
cerebral cortex
Neurons
Rats
Cultured Cells
Microscopic examination
animal communication
cultured cells
Communication
lasers
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Calcium dynamics of cortical astrocytic networks in vivo. / Hirase, Hajime; Qian, Lifen; Barthó, P.; Buzsáki, György.

In: PLoS Biology, Vol. 2, No. 4, 2004.

Research output: Contribution to journalArticle

Hirase, Hajime ; Qian, Lifen ; Barthó, P. ; Buzsáki, György. / Calcium dynamics of cortical astrocytic networks in vivo. In: PLoS Biology. 2004 ; Vol. 2, No. 4.
@article{44316d72d1264e4f82b9d65e88e716c2,
title = "Calcium dynamics of cortical astrocytic networks in vivo",
abstract = "Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca2+]i events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca2+]i events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca 2+]i activity in individual cells and a robust coordination of [Ca2+]i signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.",
author = "Hajime Hirase and Lifen Qian and P. Barth{\'o} and Gy{\"o}rgy Buzs{\'a}ki",
year = "2004",
doi = "10.1371/journal.pbio.0020096",
language = "English",
volume = "2",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "4",

}

TY - JOUR

T1 - Calcium dynamics of cortical astrocytic networks in vivo

AU - Hirase, Hajime

AU - Qian, Lifen

AU - Barthó, P.

AU - Buzsáki, György

PY - 2004

Y1 - 2004

N2 - Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca2+]i events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca2+]i events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca 2+]i activity in individual cells and a robust coordination of [Ca2+]i signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.

AB - Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous [Ca2+]i events in individual astrocytes were similar to those observed in vitro. Coordination of [Ca2+]i events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic [Ca 2+]i activity in individual cells and a robust coordination of [Ca2+]i signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.

UR - http://www.scopus.com/inward/record.url?scp=19344362473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19344362473&partnerID=8YFLogxK

U2 - 10.1371/journal.pbio.0020096

DO - 10.1371/journal.pbio.0020096

M3 - Article

C2 - 15094801

AN - SCOPUS:19344362473

VL - 2

JO - PLoS Biology

JF - PLoS Biology

SN - 1544-9173

IS - 4

ER -