Calcium-dependent protein kinase in maize and sorghum induced by polyethylene glycol

Anikó Pestenácz, L. Erdei

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Several environmental and hormonal stresses activate signal transduction in plants. Various enzymes are involved in these mechanisms. Studying one of these enzymes, we found osmotically-induced, calcium-dependent protein kinases (CDPKs), and hypothesized a connection between osmotic and hormonal signals. CDPKs were investigated in cultivars of relatively drought-tolerant maize (Zea mays L.) and drought-tolerant sorghum (Sorghum bicolor L.). Plants were grown hydroponically for 11 days and treated with abscisic acid (ABA, 0.1 μM) starting from day 5, and with polyethylene glycol 6000 starting from day 8 at 0, 100 and 200 mOsm concentrations. As a function of time, treatments lasted for 0, 15, 30, 60, 120 min and 72 h. For the determination of CDPK activity, the 25 000 g supernatant of shoots and roots were used for SDS-PAGE and autoradiography. An increased CDPK activity was found after 1 h of osmotic stress in sorghum roots and only low levels of phosphorylation could be measured in maize. Little or no activities were detected in the shoots. The Ca2+-dependent phosphorylation appeared as 49- and 52-kDa bands. Calmodulin (CaM) added in vitro did not change the enzyme activity but inhibition by a CaM antagonist, trifluoroacetic acid, was significant. We also found that pretreatment with ABA reverted the stress-induced changes in sorghum roots. We concluded that this CDPK is involved in the early steps of the signal transduction pathway, and is connected with ABA-induced mechanisms.

Original languageEnglish
Pages (from-to)360-364
Number of pages5
JournalPhysiologia Plantarum
Volume97
Issue number2
DOIs
Publication statusPublished - Jun 1996

Fingerprint

Sorghum
polyethylene glycol
Sorghum (Poaceae)
protein kinases
Zea mays
calcium
corn
abscisic acid
Droughts
Calmodulin
calmodulin
Signal Transduction
Enzymes
Phosphorylation
signal transduction
phosphorylation
Trifluoroacetic Acid
Abscisic Acid
drought
Osmotic Pressure

Keywords

  • Abscisic acid
  • calcium-dependent protein kinase
  • calmodulin
  • calmodulin antagonist
  • maize
  • osmotic stress
  • polyethylene glycol 6000
  • Sorghum bicolor
  • Zea mays

ASJC Scopus subject areas

  • Plant Science

Cite this

Calcium-dependent protein kinase in maize and sorghum induced by polyethylene glycol. / Pestenácz, Anikó; Erdei, L.

In: Physiologia Plantarum, Vol. 97, No. 2, 06.1996, p. 360-364.

Research output: Contribution to journalArticle

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