Calcium-dependent modulation of the plateau phase of action potential in isolated ventricular cells of rabbit heart

Z. Papp, N. Peineau, G. Szigeti, J. Argibay, L. Kovács

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

[Ca2+](i)-dependent modulation of the action potential has been studied in Fura-2 dialysed ventricular myocytes of the rabbit using the whole-cell current-clamp method. Fifteen consecutive action potentials (AP1-AP15) and [Ca2+](i) transients were elicited at a frequency of 0.2 Hz. A single, brief application of caffeine (during AP9) first enhanced and thereafter attenuated the [Ca2+](i) transients accompanying AP9 and AP10-AP12, respectively. This approach provided direct comparison between time courses of action potentials: during the initial steady state (e.g. AP8) and when Ca2+ release from the sarcoplasmic reticulum was increased by caffeine (AP9) or decreased by depletion (AP10). The increase in [Ca2+](i) facilitated repolarization and decreased action potential duration. However, action potentials at reduced Ca2+ release (AP10) had longer duration than during steady state. The caffeine-induced changes in L-type Ca2+ current (I(Ca,L)), during voltage-clamp conditions partially explained the effects of caffeine on action potentials. When I(Ca,L) was blocked by 500 μmol L-1 Cd2+, enhanced [Ca2+](i) transients revealed an extra current component which was outward at +10 mV and inward at the resting membrane potential (most probably the transient inward current). In the presence of Cd2+, however, AP8 and AP10 had identical time courses, suggesting that I(Ca,L) alone was responsible for the lengthening of AP10. Alterations in the transmembrane Na+ gradient resulted in changes of the steady state action potential durations (AP8) consistently with the expected modulation of the Na+-Ca2+ exchange current. However, the contribution of this current to the [Ca2+](i)-dependent behaviour of action potential plateau could not be demonstrated.

Original languageEnglish
Pages (from-to)119-129
Number of pages11
JournalActa Physiologica Scandinavica
Volume167
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Action Potentials
Rabbits
Calcium
Caffeine
Fura-2
Sarcoplasmic Reticulum
Membrane Potentials
Muscle Cells

Keywords

  • [Ca](i) transient
  • Action potential
  • Arrhythmia
  • Rabbit heart

ASJC Scopus subject areas

  • Physiology

Cite this

Calcium-dependent modulation of the plateau phase of action potential in isolated ventricular cells of rabbit heart. / Papp, Z.; Peineau, N.; Szigeti, G.; Argibay, J.; Kovács, L.

In: Acta Physiologica Scandinavica, Vol. 167, No. 2, 1999, p. 119-129.

Research output: Contribution to journalArticle

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