Calcium carbonate crystal growth beneath Langmuir monolayers of acidic β-hairpin peptides

Haofei Gong, Yi Yang, Manuela Pluntke, Othmar Marti, Zs. Majer, Norbert Sewald, Dirk Volkmer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Four amphiphilic peptides with designed hairpin structure were synthesized and their monolayers were employed as model systems to study biologically inspired calcium carbonate crystallization. Langmuir monolayers of hairpin peptides were investigated by surface pressure area isotherms, surface potential isotherms, Brewster angle microscopy (BAM), atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy. A β-hairpin conformation was found for all peptides at the air-water interface although their packing arrangements seem to be different. Crystallization of calcium carbonate under these peptide monolayers was investigated at different surface pressures and growth times both by in situ optical microscopy, BAM and ex situ investigations such as scanning electron microscopy (SEM) and transmission electron microscopy (TEM). An amorphous calcium carbonate precursor was found at the initial crystallization stage. The crystallization process occurred in three stages. It starts from the nucleation of amorphous particles being a kinetically controlled process. Crystal nuclei subsequently aggregate to large particles and vaterite crystals start to form inside the amorphous layer, with the monolayer fluidity exerting an important role. The third process includes the re-crystallization of vaterite to calcite, which is thermodynamically controlled by monolayer structural factors including the monolayer flexibility and packing arrangement of the polar headgroups. Thus, the kinetic factors, monolayer fluidity and flexibility as well as structure factors govern the crystal morphology and polymorph distribution simultaneously and synergistically.

Original languageEnglish
Pages (from-to)16857-16871
Number of pages15
JournalDalton Transactions
Volume43
Issue number44
DOIs
Publication statusPublished - Nov 28 2014

Fingerprint

Calcium Carbonate
Crystallization
Monolayers
Peptides
Microscopy
Fluidity
Crystals
Isotherms
Pressure
Atomic Force Microscopy
Fourier Transform Infrared Spectroscopy
Transmission Electron Microscopy
Electron Scanning Microscopy
Polymorphism
Optical microscopy
Conformations
Air
Atomic force microscopy
Microscopic examination
Nucleation

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Medicine(all)

Cite this

Gong, H., Yang, Y., Pluntke, M., Marti, O., Majer, Z., Sewald, N., & Volkmer, D. (2014). Calcium carbonate crystal growth beneath Langmuir monolayers of acidic β-hairpin peptides. Dalton Transactions, 43(44), 16857-16871. https://doi.org/10.1039/c4dt01154f

Calcium carbonate crystal growth beneath Langmuir monolayers of acidic β-hairpin peptides. / Gong, Haofei; Yang, Yi; Pluntke, Manuela; Marti, Othmar; Majer, Zs.; Sewald, Norbert; Volkmer, Dirk.

In: Dalton Transactions, Vol. 43, No. 44, 28.11.2014, p. 16857-16871.

Research output: Contribution to journalArticle

Gong, H, Yang, Y, Pluntke, M, Marti, O, Majer, Z, Sewald, N & Volkmer, D 2014, 'Calcium carbonate crystal growth beneath Langmuir monolayers of acidic β-hairpin peptides', Dalton Transactions, vol. 43, no. 44, pp. 16857-16871. https://doi.org/10.1039/c4dt01154f
Gong, Haofei ; Yang, Yi ; Pluntke, Manuela ; Marti, Othmar ; Majer, Zs. ; Sewald, Norbert ; Volkmer, Dirk. / Calcium carbonate crystal growth beneath Langmuir monolayers of acidic β-hairpin peptides. In: Dalton Transactions. 2014 ; Vol. 43, No. 44. pp. 16857-16871.
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