Calcium buffer proteins are specific markers of human retinal neurons

Orsolya Kántor, Szilvia Mezey, Jennifer Adeghate, Angela Naumann, Roland Nitschke, Anna Énzsöly, Arnold Szabó, Ákos Lukáts, János Németh, Zoltán Somogyvári, Béla Völgyi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ca2+-buffer proteins (CaBPs) modulate the temporal and spatial characteristics of transient intracellular Ca2+-concentration changes in neurons in order to fine-tune the strength and duration of the output signal. CaBPs have been used as neurochemical markers to identify and trace neurons of several brain loci including the mammalian retina. The CaBP content of retinal neurons, however, varies between species and, thus, the results inferred from animal models cannot be utilised directly by clinical ophthalmologists. Moreover, the shortage of well-preserved human samples greatly impedes human retina studies at the cellular and network level. Our purpose has therefore been to examine the distribution of major CaBPs, including calretinin, calbindin-D28, parvalbumin and the recently discovered secretagogin in exceptionally well-preserved human retinal samples. Based on a combination of immunohistochemistry, Neurolucida tracing and Lucifer yellow injections, we have established a database in which the CaBP marker composition can be defined for morphologically identified cell types of the human retina. Hence, we describe the full CaBP make-up for a number of human retinal neurons, including HII horizontal cells, AII amacrine cells, type-1 tyrosine-hydroxylase-expressing amacrine cells and other lesser known neurons. We have also found a number of unidentified cells whose morphology remains to be characterised. We present several examples of the colocalisation of two or three CaBPs with slightly different subcellular distributions in the same cell strongly suggesting a compartment-specific division of labour of Ca2+-buffering by CaBPs. Our work thus provides a neurochemical framework for future ophthalmological studies and renders new information concerning the cellular and subcellular distribution of CaBPs for experimental neuroscience.

Original languageEnglish
Pages (from-to)29-50
Number of pages22
JournalCell And Tissue Research
Volume365
Issue number1
DOIs
Publication statusPublished - Jul 1 2016

Keywords

  • Calbindin
  • Calretinin
  • Parvalbumin
  • Retina
  • Secretagogin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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  • Cite this

    Kántor, O., Mezey, S., Adeghate, J., Naumann, A., Nitschke, R., Énzsöly, A., Szabó, A., Lukáts, Á., Németh, J., Somogyvári, Z., & Völgyi, B. (2016). Calcium buffer proteins are specific markers of human retinal neurons. Cell And Tissue Research, 365(1), 29-50. https://doi.org/10.1007/s00441-016-2376-z