Calcium accumulation by dendritic mitochondria declines along the apical dendrites of pyramidal neurons in area CA1 of guinea pig hippocampal slices

L. Siklós, U. Kuhnt

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Abstract

An electron microscopic histochemical study was performed in stratum radiatum of area CA1 of guinea pig hippocampal slices in order to determine the spatial distribution of a dendritic mitochondrial subpopulation which accumulated calcium during in vitro incubation. A distribution gradient was found along the course of apical dendrites exhibiting the highest density values at the base of the dendrites and decaying to baseline values at about 50 μm distal from the cell body layer. The pronounced calcium accumulation by mitochondria in the proximal apical dendrites was markedly but not completely reduced by blocking L-type Ca-channels. These results (i) support the observation of a clustered distribution of L-type Ca-channels at the base of apical dendrites, (ii) designate these voltage dependent Ca2+ channels as one of the possible routes for calcium influx caused by hypoxia/ischemia induced during slice preparation, and (iii) emphasize the role of mitochondrial calcium sequestering under ischemic/hypoxic conditions.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalNeuroscience Letters
Volume173
Issue number1-2
DOIs
Publication statusPublished - May 23 1994

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Pyramidal Cells
Dendrites
Mitochondria
Guinea Pigs
Calcium
Hippocampal CA1 Region
Ischemia
Observation
Electrons

Keywords

  • Ca-uptake
  • Calcium channel
  • Electron microscopy
  • Hippocampus
  • Mitochondrion
  • Oxalate-pyroantimonate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "An electron microscopic histochemical study was performed in stratum radiatum of area CA1 of guinea pig hippocampal slices in order to determine the spatial distribution of a dendritic mitochondrial subpopulation which accumulated calcium during in vitro incubation. A distribution gradient was found along the course of apical dendrites exhibiting the highest density values at the base of the dendrites and decaying to baseline values at about 50 μm distal from the cell body layer. The pronounced calcium accumulation by mitochondria in the proximal apical dendrites was markedly but not completely reduced by blocking L-type Ca-channels. These results (i) support the observation of a clustered distribution of L-type Ca-channels at the base of apical dendrites, (ii) designate these voltage dependent Ca2+ channels as one of the possible routes for calcium influx caused by hypoxia/ischemia induced during slice preparation, and (iii) emphasize the role of mitochondrial calcium sequestering under ischemic/hypoxic conditions.",
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N2 - An electron microscopic histochemical study was performed in stratum radiatum of area CA1 of guinea pig hippocampal slices in order to determine the spatial distribution of a dendritic mitochondrial subpopulation which accumulated calcium during in vitro incubation. A distribution gradient was found along the course of apical dendrites exhibiting the highest density values at the base of the dendrites and decaying to baseline values at about 50 μm distal from the cell body layer. The pronounced calcium accumulation by mitochondria in the proximal apical dendrites was markedly but not completely reduced by blocking L-type Ca-channels. These results (i) support the observation of a clustered distribution of L-type Ca-channels at the base of apical dendrites, (ii) designate these voltage dependent Ca2+ channels as one of the possible routes for calcium influx caused by hypoxia/ischemia induced during slice preparation, and (iii) emphasize the role of mitochondrial calcium sequestering under ischemic/hypoxic conditions.

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