Calcitonin gene-related peptide-immunoreactive nerve fibres in the small intestine of the guinea-pig: electron-microscopic immunocytochemistry

E. Fehér, G. Burnstock, I. M. Varndell, J. M. Polak

Research output: Contribution to journalArticle

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Abstract

Calcitonin gene-related peptide (CGRP)-containing nerve fibres were identified by pre-and post-embedding electron-microscopic immunocytochemistry in the guinea-pig small intestine. Immunoreactive nerve processes were numerous in the mucosa and submucosa, especially in the connective tissue among the crypts of Lieberkühn. In some cases they were found in close apposition to epithelial cells. Many of the labelled nerve fibres were observed around blood vessels, especially arterioles. In the inner circular muscle layer, the immunoreactive nerve processes were found in close association (sometimes less than 40 nm) to smooth muscle cells. CGRP-positive terminals contained a predominance of electron-lucent synaptic vesicles (35-40 nm in diameter) together with a few large granular vesicles (80-120 nm in diameter). Post-embedding immunostaining, using the immunogold procedure, localized CGRP-immunoreactivity in large granular vesicles, 80-92 nm in diameter. These ultrastructural observations confirm that CGRP-containing nerve fibres exist in the small intestine and suggest that they may participate in the regulation of the smooth muscle activity, mucosal cell secretion and blood flow and, by analogy with other systems, a sensory role also seems likely.

Original languageEnglish
Pages (from-to)353-358
Number of pages6
JournalCell and Tissue Research
Volume245
Issue number2
DOIs
Publication statusPublished - Aug 1986

Fingerprint

Calcitonin Gene-Related Peptide
Nerve Fibers
Small Intestine
Guinea Pigs
Immunohistochemistry
Electrons
Muscle
Fibers
Synaptic Vesicles
Blood vessels
Arterioles
Connective Tissue
Smooth Muscle Myocytes
Smooth Muscle
Blood Vessels
Blood Cells
Mucous Membrane
Blood
Epithelial Cells
Cells

Keywords

  • Autonomic innervation
  • Calcitonin gene-related peptide (CGRP)
  • Guinea-pig
  • Small intestine

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Calcitonin gene-related peptide-immunoreactive nerve fibres in the small intestine of the guinea-pig : electron-microscopic immunocytochemistry. / Fehér, E.; Burnstock, G.; Varndell, I. M.; Polak, J. M.

In: Cell and Tissue Research, Vol. 245, No. 2, 08.1986, p. 353-358.

Research output: Contribution to journalArticle

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