Calcitonin gene-related peptide and capsaicin inhibit the circular muscle of the guinea-pig ileum

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) is known to excite, through the release of acetylcholine, the circular muscle (CM) of the guinea-pig ileum in vitro. In the present experiments, the effect of rat CGRP was investigated on the CM of tetrodotoxin-treated, spontaneously active ileum preparations. CGRP (1-10 nM) caused concentration-dependent inhibition of both the amplitude and frequency of spontaneous CM contractions. Capsaicin (a sensory stimulant known to release CGRP from primary afferents) also inhibited CM activity. The effect of 1 μM capsaicin underwent rapid desensitization, indicating specific action on afferent structures, whereas a high concentration of the drug (33 μM) inhibited CM activity most probably on the smooth muscle itself.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalRegulatory Peptides
Volume35
Issue number1
DOIs
Publication statusPublished - Jul 23 1991

Fingerprint

Calcitonin Gene-Related Peptide
Capsaicin
Ileum
Muscle
Guinea Pigs
Muscles
Tetrodotoxin
Muscle Contraction
Acetylcholine
Smooth Muscle
Rats
Pharmaceutical Preparations
Experiments

Keywords

  • Enteric neuropeptide
  • Gut motility
  • Primary afferent
  • Tetrodotoxin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Calcitonin gene-related peptide and capsaicin inhibit the circular muscle of the guinea-pig ileum. / Barthó, L.

In: Regulatory Peptides, Vol. 35, No. 1, 23.07.1991, p. 43-48.

Research output: Contribution to journalArticle

@article{f5963b4ea32d459591850c170cc831e5,
title = "Calcitonin gene-related peptide and capsaicin inhibit the circular muscle of the guinea-pig ileum",
abstract = "Calcitonin gene-related peptide (CGRP) is known to excite, through the release of acetylcholine, the circular muscle (CM) of the guinea-pig ileum in vitro. In the present experiments, the effect of rat CGRP was investigated on the CM of tetrodotoxin-treated, spontaneously active ileum preparations. CGRP (1-10 nM) caused concentration-dependent inhibition of both the amplitude and frequency of spontaneous CM contractions. Capsaicin (a sensory stimulant known to release CGRP from primary afferents) also inhibited CM activity. The effect of 1 μM capsaicin underwent rapid desensitization, indicating specific action on afferent structures, whereas a high concentration of the drug (33 μM) inhibited CM activity most probably on the smooth muscle itself.",
keywords = "Enteric neuropeptide, Gut motility, Primary afferent, Tetrodotoxin",
author = "L. Barth{\'o}",
year = "1991",
month = "7",
day = "23",
doi = "10.1016/0167-0115(91)90252-C",
language = "English",
volume = "35",
pages = "43--48",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Calcitonin gene-related peptide and capsaicin inhibit the circular muscle of the guinea-pig ileum

AU - Barthó, L.

PY - 1991/7/23

Y1 - 1991/7/23

N2 - Calcitonin gene-related peptide (CGRP) is known to excite, through the release of acetylcholine, the circular muscle (CM) of the guinea-pig ileum in vitro. In the present experiments, the effect of rat CGRP was investigated on the CM of tetrodotoxin-treated, spontaneously active ileum preparations. CGRP (1-10 nM) caused concentration-dependent inhibition of both the amplitude and frequency of spontaneous CM contractions. Capsaicin (a sensory stimulant known to release CGRP from primary afferents) also inhibited CM activity. The effect of 1 μM capsaicin underwent rapid desensitization, indicating specific action on afferent structures, whereas a high concentration of the drug (33 μM) inhibited CM activity most probably on the smooth muscle itself.

AB - Calcitonin gene-related peptide (CGRP) is known to excite, through the release of acetylcholine, the circular muscle (CM) of the guinea-pig ileum in vitro. In the present experiments, the effect of rat CGRP was investigated on the CM of tetrodotoxin-treated, spontaneously active ileum preparations. CGRP (1-10 nM) caused concentration-dependent inhibition of both the amplitude and frequency of spontaneous CM contractions. Capsaicin (a sensory stimulant known to release CGRP from primary afferents) also inhibited CM activity. The effect of 1 μM capsaicin underwent rapid desensitization, indicating specific action on afferent structures, whereas a high concentration of the drug (33 μM) inhibited CM activity most probably on the smooth muscle itself.

KW - Enteric neuropeptide

KW - Gut motility

KW - Primary afferent

KW - Tetrodotoxin

UR - http://www.scopus.com/inward/record.url?scp=0025912882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025912882&partnerID=8YFLogxK

U2 - 10.1016/0167-0115(91)90252-C

DO - 10.1016/0167-0115(91)90252-C

M3 - Article

VL - 35

SP - 43

EP - 48

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1

ER -